Abstract
A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned into prokaryotic expression vector pTrcHisA, and transformed into Escherichia coli strain Rosetta (DE3). Less than 4mg of the desired protein/l could be obtained in the form of inclusion bodies. Of various inducers and enhancers of stress responses, the heat-shock enhances, streptomycin, the osmotic stress inducers, d-arabinose and sucrose, and the cold-shock enhancer, tetracycline, simulated the expression of the antibody-targeted plasminogen activator by 2- 5-fold.
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Revisions requested 7 July 2004; Revisions received 3 September 2004
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Yu, M., Yu, M. & Ru, B. Expression of an antibody-targeted plasminogen activator in Escherichia coli using chemically induced stress responses. Biotechnol Lett 26, 1629–1634 (2004). https://doi.org/10.1007/s10529-004-3185-0
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DOI: https://doi.org/10.1007/s10529-004-3185-0