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Open AccessNR4A1 deletion promotes pro-angiogenic polarization of macrophages derived from classical monocytes in a mouse model of neovascular age-related macular degeneration
Neovascular age-related macular degeneration causes vision loss from destructive angiogenesis, termed choroidal neovascularization (CNV). Cx3cr1−/− mice display alterations in non-classical monocytes and microgli...
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Open AccessAutomating iPSC generation to enable autologous photoreceptor cell replacement therapy
Inherited retinal degeneration is a leading cause of incurable vision loss in the developed world. While autologous iPSC mediated photoreceptor cell replacement is theoretically possible, the lack of commercia...
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Open AccessCD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells
Regulatory T (Treg) cells are one of the major immunosuppressive cell types in cancer and a potential target for immunotherapy, but targeting tumor-infiltrating (TI) Treg cells has been challenging. Here, usin...
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Open AccessMap** the immune environment in clear cell renal carcinoma by single-cell genomics
Clear cell renal cell carcinoma (ccRCC) is one of the most immunologically distinct tumor types due to high response rate to immunotherapies, despite low tumor mutational burden. To characterize the tumor immu...
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Open AccessStepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells
Endothelial cells (ECs) are essential regulators of the vasculature, lining arteries, veins, and capillary beds. While all ECs share a number of structural and molecular features, heterogeneity exists dependin...
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Open AccessA simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells
The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gai...
