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Article
Open AccessRestoration of high-sensitivity and adapting vision with a cone opsin
Inherited and age-related retinal degenerative diseases cause progressive loss of rod and cone photoreceptors, leading to blindness, but spare downstream retinal neurons, which can be targeted for optogenetic ...
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Article
Open AccessThe flagellin of candidate live biotherapeutic Enterococcus gallinarum MRx0518 is a potent immunostimulant
Many links between gut microbiota and disease development have been established in recent years, with particular bacterial strains emerging as potential therapeutics rather than causative agents. In this study...
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Article
Open AccessAuthor Correction: Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor
Kevin J. Cao and Richard H. Kramer, who developed extended release with beta cyclodextrin, were inadvertently omitted from the author list and author contributions section of this Article. These errors have no...
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Protocol
Optogenetic Retinal Gene Therapy with the Light Gated GPCR Vertebrate Rhodopsin
In retinal disease, despite the loss of light sensitivity as photoreceptors die, many retinal interneurons survive in a physiologically and metabolically functional state for long periods. This provides an opp...
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Article
Open AccessRestoration of patterned vision with an engineered photoactivatable G protein-coupled receptor
Retinitis pigmentosa results in blindness due to degeneration of photoreceptors, but spares other retinal cells, leading to the hope that expression of light-activated signaling proteins in the surviving cells...
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Article
Large-scale discovery of enhancers from human heart tissue
Len Pennacchio, Axel Visel and colleagues use an epigenomic approach to identify a large number of candidate enhancers from human heart tissue. This work will facilitate further studies into the role of enhanc...
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Article
ChIP-Seq identification of weakly conserved heart enhancers
Len Pennacchio and colleagues used ChIP-Seq with the enhancer-associated protein p300 to identify 3,000 candidate cardiac transcriptional enhancers in embryonic mice at E11.5. Notably, most candidate heart enh...
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Article
ChIP-seq accurately predicts tissue-specific activity of enhancers
A major yet unresolved quest in decoding the human genome is the identification of the regulatory sequences that control the spatial and temporal expression of genes. Distant-acting transcriptional enhancers a...
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Article
Ultraconservation identifies a small subset of extremely constrained developmental enhancers
Extended perfect human-rodent sequence identity of at least 200 base pairs (ultraconservation) is potentially indicative of evolutionary or functional uniqueness. We used a transgenic mouse assay to compare th...
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Article
In vivo enhancer analysis of human conserved non-coding sequences
Identifying the non-coding DNA sequences that act at a distance to regulate patterns of gene expression is not a simple matter; one useful pointer is evolutionary sequence conservation. An in vivo analysis of 167...
