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Chapter
Mechanism of Regulation of Gene Expression for Chloroplast Proteins
Both plastid- and nuclear-encoded proteins are involved in photosynthetic reactions carried out by chloroplasts. While the structure of various plastome-encoded photosynthesis-related genes has been worked out...
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Chapter
Engineering Herbicide Resistance Pathways in Plastids
Herbicides are an integral part of modern day agriculture as they facilitate efficient crop management. Most of the herbicides target specific enzymes involved in metabolic pathways that are vital for plant gr...
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Protocol
Chloroplast Genetic Engineering to Improve Agronomic Traits
Major crop losses occur annually as a result of biotic and abiotic stresses. The ability to hyperexpress foreign proteins, single-step multigene engineering, lack of positive effect and gene silencing, vector ...
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Article
Stable transformation of the cotton plastid genome and maternal inheritance of transgenes
Chloroplast genetic engineering overcomes concerns of gene containment, low levels of transgene expression, gene silencing, positional and pleiotropic effects or presence of vector sequences in transformed gen...
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Article
Open AccessASAP: Amplification, sequencing & annotation of plastomes
Availability of DNA sequence information is vital for pursuing structural, functional and comparative genomics studies in plastids. Traditionally, the first step in mining the valuable information within a chl...
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Protocol
Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis
Chloroplast genetic engineering offers a number of unique advantages, including high-level transgene expression, multigene engineering in a single transformation event, transgene containment via maternal inher...
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Article
Open AccessRapid and accurate pyrosequencing of angiosperm plastid genomes
Plastid genome sequence information is vital to several disciplines in plant biology, including phylogenetics and molecular biology. The past five years have witnessed a dramatic increase in the number of comp...
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Article
Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation
Cultivated strawberry (Fragaria ×ananassa) is a valuable crop, yet the absence of a rapid, high-throughput transgenic system has precluded meaningful application of biotechnology and translation of information fr...
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Article
Invited review: Transformation of strawberry: The basis for translational genomics in Rosaceae
Translational genomics is defined as the application of molecular-genetic principles derived from model systems to species of experimental or economic interest. The past 20 years of research in plant model sys...
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Article
Open AccessComparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp. ancestrale– A wild ancestor of cultivated buckwheat
Chloroplast genome sequences are extremely informative about species-interrelationships owing to its non-meiotic and often uniparental inheritance over generations. The subject of our study, Fagopyrum esculentum,...
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Chapter
Rosaceaous Genome Sequencing: Perspectives and Progress
The long-term goal of plant genomics is to identify, isolate and determine the function of plant genes that are associated with both vegetative and reproductive phenotypes. Most phenotypes require the coordina...
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Article
The genome of the domesticated apple (Malus × domestica Borkh.)
Riccardo Velasco and colleagues report the genome sequence of the 'Golden Delicious' domesticated apple. These data shed new insight into the genomic events that preceded the origin of this crop.
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Article
In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide
To study the developmental transition of chloroplasts from C3 to C4 photosynthesis in the terrestrial single-cell C4 species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material de...
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Article
Open AccessWhole genome sequencing of peach (Prunus persica L.) for SNP identification and selection
The application of next generation sequencing technologies and bioinformatic scripts to identify high frequency SNPs distributed throughout the peach genome is described. Three peach genomes were sequenced usi...
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Article
Open AccessRapid gene-based SNP and haplotype marker development in non-model eukaryotes using 3'UTR sequencing
Sweet cherry (Prunus avium L.), a non-model crop with narrow genetic diversity, is an important member of sub-family Amygdoloideae within Rosaceae. Compared to other important members like peach and apple, sweet ...
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Article
Effect of phyllotactic position and cultural treatments toward successful direct shoot organogenesis in dwarf ‘Pixie’ grapevine (Vitis vinifera L.)
In Vitis spp. where somatic embryogenesis-based regeneration predominates, an efficient, reproducible and robust method of direct shoot organogenesis from leaf explant material has been established in the dwarf w...
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Article
Rootstock scion somatogenetic interactions in perennial composite plants
The ancient plant production practice of grafting which instantly imparts new physiological properties to the desirable scion still remains shrouded in mystery. Yet, grafting remains a widely used technique in...
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Article
Inactivation of Escherichia coli Population on Fruit Surfaces Using Ultraviolet-C Light: Influence of Fruit Surface Characteristics
Ultraviolet-C (UV-C 254 nm) light is a possible alternative for chemical disinfection of fresh fruits. However, studies on the influence of surface characteristics on the kinetics of UV-C inactivation of micro...
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Article
Antisense expression of peach mildew resistance locus O (PpMlo1) gene confers cross-species resistance to powdery mildew in Fragaria x ananassa
Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment....
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Article
Evaluation of pea accessions and commercial cultivars for fusarium root rot resistance
Fusarium root rot caused by Fusarium solani f. sp. pisi (Fsp) can result in major yield losses in pea (Pisum sativum L.). Currently no fungicides effectively manage this disease. Previous studies evaluated the Pi...
