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Article
Rapid alkalinization factor 22 has a structural and signalling role in root hair cell wall assembly
Pressurized cells with strong walls make up the hydrostatic skeleton of plants. Assembly and expansion of such stressed walls depend on a family of secreted RAPID ALKALINIZATION FACTOR (RALF) peptides, which b...
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Protocol
Noninvasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors
In this protocol, we present a noninvasive in planta bioimaging technique for the analysis of hydrogen peroxide (H2O2) and glutathione redox potential in adult Arabidopsis thaliana plants. The technique is based ...
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Article
Open AccessThree-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction
Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. O...
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Article
The Arabidopsis thaliana transcription factor MYB59 regulates calcium signalling during plant growth and stress response
Transcription factor MYB59 is involved in plant growth and stress responses by acting as negative regulator of Ca signalling and homeostasis.
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Protocol
In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana
Calcium imaging in plants requires a high-resolution microscope, able to perform volumetric acquisition in a few seconds, inducing as low photobleaching and phototoxicity as possible to the sample. Light sheet...
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Article
Open AccessUnique resistance traits against downy mildew from the center of origin of grapevine (Vitis vinifera)
The Eurasian grapevine (Vitis vinifera), an Old World species now cultivated worldwide for high-quality wine production, is extremely susceptible to the agent of downy mildew, Plasmopara viticola. The cultivation...
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Article
Trans-splicing of plastid rps12 transcripts, mediated by AtPPR4, is essential for embryo patterning in Arabidopsis thaliana
AtPPR4-mediated trans-splicing of plastid rps12 transcripts is essential for key embryo morphogenetic events such as development of cotyledons, determination of provascular tissue, and organization of the shoot a...
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Protocol
Calcium Ion Dynamics in Roots: Imaging and Analysis
Calcium sensors are indispensable tools to study the role of Ca2+ and visualize Ca2+ dynamics during biological processes. Over the past years, the field of Ca2+ imaging has strongly expanded by the development o...
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Protocol
In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB)
Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).
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Article
Open AccessCorrection: Corrigendum: Constitutive cyclic GMP accumulation in Arabidopsis thaliana compromises systemic acquired resistance induced by an avirulent pathogen by modulating local signals
Scientific Reports 6: Article number: 36423; published online: 04 November 2016; updated: 03 May 2017. This Article contains an error in Figure 3c, where the y-axis ‘ASC + DHA (μmol/mg prot)’ is incorrectly gi...
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Article
Open AccessConstitutive cyclic GMP accumulation in Arabidopsis thaliana compromises systemic acquired resistance induced by an avirulent pathogen by modulating local signals
The infection of Arabidopsis thaliana plants with avirulent pathogens causes the accumulation of cGMP with a biphasic profile downstream of nitric oxide signalling. However, plant enzymes that modulate cGMP level...
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Article
Open AccessOsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress
Na+ exclusion from leaf blades is one of the key mechanisms for glycophytes to cope with salinity stress. Certain class I transporters of the high-affinity K+ transporter (HKT) family have been demonstrated to me...
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Article
OsHKT2;2/1-mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress
HKT transporters are Na+-permeable membrane proteins, which mediate Na+ and K+ homeostasis in K+-depleted and saline environments in plants. Class II HKT transporters, a distinct subgroup found predominantly in m...
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Protocol
Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors
Mitochondrial physiology sets the basis for function of the organelle and vice versa. While a limited range of in vivo parameters, such as oxygen consumption, has been classically accessible for measurement, a...
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Article
The phosphoinositide PI(3,5)P2 mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells
Two-pore channel proteins (TPC) encode intracellular ion channels in both animals and plants. In mammalian cells, the two isoforms (TPC1 and TPC2) localize to the endo-lysosomal compartment, whereas the plant ...
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Article
Open AccessProduction of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinereaare preceded and depend on a burst of calcium
Wounded leaves of Arabidopsis thaliana produce reactive oxygen species (ROS) within minutes after wounding and become resistant to the pathogenic fungus Botrytis cinerea at a local level. This fast response of th...
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Article
Open AccessPerception of soft mechanical stress in Arabidopsis leaves activates disease resistance
In a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed...
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Chapter
Peroxisome Ca2+ Homeostasis in Animal and Plant Cells
Ca2+ homeostasis in peroxisomes has been an unsolved problem for many years. Recently novel probes to monitor Ca2+ levels in the lumen of peroxisomes in living cells of both animal and plant cells have been devel...
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Article
Open AccessTwo putative-aquaporin genes are differentially expressed during arbuscular mycorrhizal symbiosis in Lotus japonicus
Arbuscular mycorrhizas (AM) are widespread symbioses that provide great advantages to the plant, improving its nutritional status and allowing the fungus to complete its life cycle. Nevertheless, molecular mec...
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Article
The p23 co-chaperone protein is a novel substrate of CK2 in Arabidopsis
The ubiquitous Ser/Thr protein kinase CK2, which phosphorylates hundreds of substrates and is essential for cell life, plays important roles also in plants; however, only few plant substrates have been identif...
