24 Result(s)
within
Alan D. W. Dobson
English
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Article
Extracellular lignin and manganese peroxidase production by the white-rot fungus Coriolus versicolor 290
Lignin peroxidase (LiP) and manganese peroxidase (MnP) activity in Coriolus versicolor 290 reach maximal levels after 8 and 10 days growth respectively. Increases in LiP activity were observed upon supplementatio...
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Article
PCR amplification of lignin peroxidase genes in white rot fungi
Degenerate oligonucleotides encoding the two conserved histidine regions of the Phanerochaete chrysosporium BKM-F-1767 lignin peroxidase gene have been used as PCR primers to clone lignin peroxidase genes from th...
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Article
Oxidation of fluorene and phenanthrene by Mn(II) dependent peroxidase activity in whole cultures of Trametes(coriolus) versicolor
Manganese peroxidase (MnP) activity in 10 day old cultures of Trametes versicolor290 increased from 13 to 104 nmol/ml.min when the nitrogen concentration in the growth medium was increased from 0.1 to 0.4 gL-1 an...
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Protocol
Microbial Degradation of Alkenylbenzenes
Alkenylbenzenes, such as α-methylstyrene,β-methylstyrene, and styrene, are produced in large quantities by the petrochemical industry (Fig. 1). The majority of studies have concentrated on the simplest of all alk...
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Protocol
Generation of Species Specific DNA Probes for the Lignin Peroxidase Genes of White Rot Fungi
White rot fungi are the only known organisms capable of completely degrad- ing lignin to CO2 and H2O (see Chapter 1). Lignin, a complex three-dimen- sional molecule, is the most abundant aromatic polymer in the b...
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Article
Extracellular ligninolytic enzyme production and polymeric dye decolourization in immobilized cultures of Chrysosporium lignorum CL1
Decolourization of Poly R-478 and Poly S-119 by Chrysosporium lignorum was indicative of lignin peroxidase and manganese peroxidase activity when the fungus was cultured under static conditions or immobilized ...
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Article
Fluoranthene degradation in Pseudomonas alcaligenes PA-10
Pseudomonas alcaligenes strain PA-10 degrades thefour-ring polycyclic aromatic hydrocarbon fluoranthene, co-metabolically. HPLC analysisof the growth medium identified four intermediates, 9-fluorenone-1-carboxyl...
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Article
Effect of surfactants on fluoranthene degradation by Pseudomonas alcaligenes PA-10
Two surfactants, Tween 80 and JBR, were investigated for their effect on fluoranthene degradation by a Pseudomonad. Both surfactants enhanced fluoranthene degradation by Pseudomonas alcaligenes PA-10 in shake fla...
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Article
Metagenomic approaches to exploit the biotechnological potential of the microbial consortia of marine sponges
Natural products isolated from sponges are an important source of new biologically active compounds. However, the development of these compounds into drugs has been held back by the difficulties in achieving a...
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Article
Isolation and Analysis of Bacteria with Antimicrobial Activities from the Marine Sponge Haliclona simulans Collected from Irish Waters
Samples of the marine sponge Haliclona simulans were collected from Irish coastal waters, and bacteria were isolated from these samples. Phylogenetic analyses of the cultured isolates showed that four different b...
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Article
Phylogenetic Diversity and Antimicrobial Activities of Fungi Associated with Haliclona simulans Isolated from Irish Coastal Waters
The diversity and antimicrobial activities of 80 fungi isolated from Haliclona simulans were assessed using different fungal media containing either agar or gellum gum. In total, 19 different genotypes were detec...
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Article
Pyrosequencing Reveals Diverse and Distinct Sponge-Specific Microbial Communities in Sponges from a Single Geographical Location in Irish Waters
Marine sponges are host to numerically vast and phylogenetically diverse bacterial communities, with 26 major phyla to date having been found in close association with sponge species worldwide. Analyses of the...
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Article
In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans
Several different methods were compared to recover a significant quantity of high quality RNA from H. simulans containing RNA of marine-derived fungi. Further processing of the RNA showed that amplification produ...
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Living Reference Work Entry In depth
Marine Invertebrate Animal Metagenomics: Porifera
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Reference Work Entry In depth
Marine Invertebrate Animal Metagenomics: Porifera
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Chapter
Marine Sponges – Molecular Biology and Biotechnology
Marine sponges are an ancient and diverse animal phylum that host well-established symbiotic microbial communities. The vast majority of the microbial genetic diversity in sponges is, however, currently inacce...
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Article
Erratum to: Deciphering the role of coumarin as a novel quorum sensing inhibitor suppressing virulence phenotypes in bacterial pathogens
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Chapter
Metagenomics as a Tool for Biodiscovery and Enhanced Production of Marine Bioactives
The application of metagenomics technologies to the area of marine bioprospecting and biodiscovery has seen a major advance in our capacity to harness the bioactive potential of the ocean, not least when we co...
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Article
Open AccessDegradation intermediates of polyhydroxy butyrate inhibits phenotypic expression of virulence factors and biofilm formation in luminescent Vibrio sp. PUGSK8
Luminescent vibrios are ubiquitous in the marine environment and are the causative agents of vibriosis and mass mortality in many aquatic animals. In aquatic environments, treatments cannot be limited to the d...
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Chapter
Psychrophiles as a Source of Novel Antimicrobials
Cold environments such as Arctic and Antarctic regions and the deep sea are richly populated by microbes which encounter the same selective pressures and/or even more than their counterparts from moderate or w...
