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Article
Open AccessCombining elemental and immunochemical analyses to characterize diagenetic alteration patterns in ancient skeletal remains
Bones and teeth are biological archives, but their structure and composition are subjected to alteration overtime due to biological and chemical degradation postmortem, influenced by burial environment and condit...
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Article
Chemiluminescence Biosensor for Non-invasive Crew Health Monitoring at the International Space Station
Long-duration manned space missions require that astronauts be equipped with analytical devices suitable for performing on-site clinical-chemistry analysis. In the frame of the “IN SITU Bioanalysis” project, a...
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Chapter and Conference Paper
On-Glass Integration of Thin Film Devices for Monitoring of Cell Bioluminescence
This reports the development of a miniaturized lab-on-glass,suitable for the on-chip detection of living cell and their on-chip thermal treatments. The glass substrate hosts, on one side, hydrogenated si...
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Article
The scope of Analytical and Bioanalytical Chemistry (ABC)
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Article
A new sensitive and quantitative chemiluminescent assay to monitor intracellular xanthine oxidase activity for rapid screening of inhibitors in living endothelial cells
Xanthine oxidase (XO) is an important enzyme, expressed at high levels in the vasculature in endothelial cells, that catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Excessive...
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Chapter
Luciferase Genes as Reporter Reactions: How to Use Them in Molecular Biology?
The latest advances in molecular biology have made available several biotechnological tools that take advantage of the high detectability and quantum efficiency of bioluminescence (BL), with an ever-increasing...
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Chapter and Conference Paper
Chemiluminescence-Based Micro-Total-Analysis System with Amorphous Silicon Photodiodes
In this paper we present a chemiluminescence-based micro-total-analysis system integrating amorphous silicon for on-chip detection as a technically feasible solution to develop “true” lab-on-chip systems, inte...
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Article
Single and multiplexed immunoassays for the chemiluminescent imaging detection of animal glues in historical paint cross-sections
The characterization of the organic components in a complex, multilayered paint structure is fundamental for studying painting techniques and for authentication and restoration purposes. Proteinaceous material...
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Article
Spatial distributions of the red palm mite, Raoiella indica (Acari: Tenuipalpidae) on coconut and their implications for development of efficient sampling plans
The red palm mite (Raoiella indica), an invasive pest of coconut, entered the Western hemisphere in 2004, then rapidly spread through the Caribbean and into Florida, USA. Develo** effective sampling methods may...
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Article
Tear proteomics in evaporative dry eye disease
To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects.
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Article
Multiplex chemiluminescence microscope imaging of P16INK4A and HPV DNA as biomarker of cervical neoplasia
Classification of cervical intraepithelial neoplasia (CIN) lesions in low-grade (CIN1) or high-grade (CIN2-3) ones is crucial for optimal patient management, but current histological diagnosis on bioptic sampl...
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Chapter and Conference Paper
Patient Dose Versus Image Quality in Digital Mammography at Instituto Português de Oncologia de Coimbra (IPOC) – Portugal
In the scope of the optimization of patient protection process (ALARA concept), the assessment of the patient radiation dose is not enough; image quality must be taken into consideration. The aim of this work ...
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Article
Railway Train-Track Dynamics for Wheelflats with Improved Contact Models
A variety of methods have been proposed to calculate the dynamic response caused by a railway vehicle affected by a wheelflat. Most of the sophisticated procedures evaluate the elastic properties of the wheel-...
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Article
Development and validation of a sensitive and fast chemiluminescent enzyme immunoassay for the detection of genetically modified maize
Proteins from the Cry 1 family, in particular Cry 1Ab, are commonly expressed in genetically modified Bt maize in order to control chewing insect pests. A sensitive chemiluminescent sandwich enzyme immunoassay...
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Article
Leaf pubescence mediates the abundance of non-prey food and the density of the predatory mite Typhlodromus pyri
Plants with leaves having numerous trichomes or domatia frequently harbor greater numbers of phytoseiid mites than do plant with leaves that lack these structures. We tested the hypothesis that this pattern oc...
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Chapter
Luminescence for the Analysis of Organic Compounds in Natural Waters
Many widely used synthetic chemicals, as well as industrial by-products, are continuously emitted into the environment. Even if present in low quantities, some of these substances or their degradation products...
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Article
Leaf pubescence and two-spotted spider mite webbing influence phytoseiid behavior and population density
Phytoseiid mites, both in agricultural and natural systems, can play an important role in the regulation of herbivorous mites. Host plant traits, such as leaf pubescence, may influence the dynamics between pre...
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Article
Analytical performance of luminescent immunoassays of different format for serum daidzein analysis
Two sensitive competitive-type solid-phase immunoassays for serum daidzein analysis have been developed and optimized. The first is a chemiluminescent enzyme immunoassay that uses black polystyrene microtiter...
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Article
Trichomes and spider-mite webbing protect predatory mite eggs from intraguild predation
Predaceous arthropods are frequently more abundant on plants with leaves that are pubescent or bear domatia than on plants with glabrous leaves. We explored the hypothesis that for some predatory mites this i...
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Article
Bio- and chemiluminescence in bioanalysis
Analytical chemiluminescence and bioluminescence represent a versatile, ultrasensitive tool with a wide range of applications in diverse fields such as biotechnology, pharmacology, molecular biology, clinical...