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Article
Selective killing of smooth muscle cells in culture by the ricin A-chain conjugated with monoclonal antibodies to a cell surface antigen via a dextran bridge
Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction o...
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Contributory presentations/posters
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Article
Intracellular Antibodies Do not Affect Transport of Protein Toxins
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Article
Ricin and Viscumin Bind to Different Sites of the Cell Membrane
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Article
A New Antigenic Epitope Appears in the Catalytic Subunit of Viscumin during Intracellular Transport
The plant toxin viscumin (60 kD) consists of B- (“binding”) and A- (“active”) subunits joined by a disulfide bond. The B-subunit is a lectin interacting with galactose-containing glycolipids and glycoproteins ...
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Article
An Epitope of the Viscumin Catalytic Subunit Exposed on Its Interaction with Lipid Bilayer
It was previously shown that the catalytic subunit of the plant toxin viscumin induces aggregation of small unilamellar liposomes and this process is inhibited by the mab_TA7 monoclonal antibody produced to th...
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Article
Monovalent and Multivalent Binding of Streptavidin to Biotinylated Gramicidin Affects the Kinetic Properties of the Ion Channel
Biotin–avidin (or streptavidin) high affinity binding has been widely applied as a universal tool for basic research as well as diagnostic and therapeutic purposes. Here we studied the interaction of streptavi...
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Article
Cloning and Expression of Catalytic Subunit of MLIII, the Ribosome-Inactivating Protein from Viscum album
We have cloned the gene encoding a precursor of mistletoe (Viscum album) toxin MLIII. Analyses of nucleotide and deduced amino acid sequences of this gene revealed significant differences between MLI and MLIII pr...
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Article
Cloning and expression of mistletoe lectin III B-subunit
Aqueous extracts of mistletoe (Viscum album L.) contain toxic proteins (lectins) MLI (viscumin), MLII, and MLIII. We previously cloned the gene encoding MLIII precursor. In the present study, a gene fragment enco...
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Article
A study of E. coli and T. maritima ribosomes by atomic force microscopy
Whole 70S ribosomes and 50S and 30S ribosomal subunits of E. coli and T. maritima were studied by atomic force microscopy. Adsorption of the ribosomal subunits on a substrate revealed considerable heterogeneity o...
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Article
Effect of functional catechol-O-methyltransferase Val158Met polymorphism on physical aggression
Genetic and psychological analysis of the relationships between catechol-O-methyltransferase Val158Met polymorphism and various types of aggressiveness was performed in 114 women. Dispersion analysis revealed ...
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Article
Micro-and nanostructural characteristics of 3D porous carriers ElastoPHB®-3D
Microstructure of the surface and micro-and nanostructure of the internal surface of 3D porous carrier ElastoPHB®-3D were studied by methods of electron microscopy and atomic force microscopy. Biological properti...
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Article
Role of renin-angiotensin system in the formation of emotional state in humans
We studied the effect of angiotensin-converting enzyme gene polymorphism on human emotional state in humans (189 athletes and 212 volunteers not engaged in sport activity). The distribution of angiotensin-conv...
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Prospects of studying the polymorphisms of key genes of neurotransmitter systems: II. The serotonergic system
The review deals with the genetic principles of personality features that are helpful in both efficient athlete selection and improved training. The variations of the genes of the serotonergic system, associat...
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Free testosterone as marker of adaptation to medium-intensive exercise
A 4-week study of adaptation reserves of the body was carried out during medium intensive exercise (medium intensive training: 60–80% threshold anaerobic metabolism). Two groups of athletes were singled out by...
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Article
Relationship between the Degree of Cardiovascular Adaptation and Th1/Th2 Polarization of Immune Response
The main parameters of humoral immunity during medium intensity exercise were studied in oarsmen with high and low cardiovascular adaptation. A relationship between high cardiovascular adaptation to exercise a...
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Article
Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise
We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90...
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Article
The origin of eubacteria with three L7/L12 protein dimers in the ribosome
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Article
Genetic Markers of Predisposition to Increased Anxiety
Associations of functional polymorphism in genes of dopamine receptor DRD2, dopamine transporter DAT, and dopamine degrading enzyme COMT with variations in anxiety sensitivity threshold were studied. On the ba...
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Article
Modulation of cardiac rhythm in the humans exposed to extremely weak alternating magnetic fields
The effect of extremely weak alternating magnetic fields of various types with the amplitudes δ of 2 μT on the heart rate variability in humans was studied. Volunteers were placed in a large-volume coil system...
