26 Result(s)

Article

Chromosome imaging by atomic force microscopy: influencing factors and comparative evaluation

Yangzhe Wu, Jiye Cai, Longqiu Cheng, Chenxi Wang, Yong Chen in Journal of Genetics (2006)

Article

High-level expression of a functional humanized single-chain variable fragment antibody against CD25 in Pichia pastoris

CD25 is a marker molecule expressed on many T and B cell neoplasms and on activated T cells that mediate organ transplant rejection and many autoimmune diseases. Single-chain variable fragments (scFvs) have ma...

Lin Wan, Huawei Cai, Hao Yang, Yanrong Lu… in Applied Microbiology and Biotechnology (2008)

Article

High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris

The administration of antibodies against the cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a promising approach in the upregulation of immune responses in many cancers and infectious diseases. The si...

Huawei Cai, Lihong Chen, Lin Wan, Lingyu Zeng… in Applied Microbiology and Biotechnology (2009)

Article

Effect of Hypoxia-inducible Factor-1α Silencing on the Sensitivity of Human Brain Glioma Cells to Doxorubicin and Etoposide

Multidrug resistance (MDR) is a significant problem underlying the poor prognosis associated with gliomas. Hypoxia-inducible factor-1α (HIF-1α) is thought to induce the genes expression involved in MDR. To eva...

Lei Chen, Peimin Feng, Shengfu Li, Dan Long, **gqiu Cheng… in Neurochemical Research (2009)

Article

Production and characterization of LEA29Y, a variant of cytotoxic T-lymphocyte antigen 4-immunoglobulin, in Pichia pastoris

Blocking the CD28/B7 costimulatory pathway is a promising strategy in the treatment of graft rejection, graft-versus-host disease and autoimmune diseases. LEA29Y, a high-affinity variant of cytotoxic T-lymphoc...

Lin Wan, Shengyun Zhu, Yingying Li, Shan Liu… in Applied Microbiology and Biotechnology (2011)

Article

A novel anti-lymphoma protein RE26 from Rozites emodensis (Berk.) Moser

A novel antitumor protein, designated RE26, with anti-lymphoma activity was purified from a Tris–HCl buffer extract of Rozites emodensis (Berk.) Moser by three successive steps of ion exchange chromatography. SDS...

Meng Gong, Minjie **a, **g Zhang, Jiao Gu… in Applied Microbiology and Biotechnology (2012)

Article

Production of bioactive, SUMO-modified, and native-like TNF-α of the rhesus monkey, Macaca mulatta, in Escherichia coli

Biotechnologically produced tumor necrosis factor alpha (TNF-α) neutralizing agents have proven efficient in patients suffering from disparate autoimmune diseases. The rhesus monkey (Macaca mulatta) could be deve...

Dianlong Jia, Hao Yang, Lin Wan, **gqiu Cheng… in Applied Microbiology and Biotechnology (2012)

Article

Production and characterization of a CD25-specific scFv-Fc antibody secreted from Pichia pastoris

Antibodies against CD25 would be novel tools for the diagnosis and treatment of adult T cell leukemia lymphoma (ATLL) and many other immune disorders. In our previous work, we successfully produced the single-...

Lin Wan, Shengyun Zhu, Jian Zhu, Hao Yang… in Applied Microbiology and Biotechnology (2013)

Article

Production and characterization of human soluble CD83 fused with the fragment crystallizable region of human IgG1 in Pichia pastoris

The cell surface protein CD83 belongs to the immunoglobulin superfamily and is highly expressed on mature dendritic cells. The soluble form of CD83, sCD83, is a potential immune suppressor. In a previous study...

Yujia Yuan, Lin Wan, Younan Chen, Meimei Shi… in Applied Microbiology and Biotechnology (2013)

Article

Assessment of early renal damage in diabetic rhesus monkeys

The objectives of the study were to improve the model system of diabetic nephropathy in nonhuman primates and assess the early renal damage. Diabetes was induced in monkeys by streptozotocin, and the animals ...

Dan Wang, **g** Liu, Sirong He, Chengshi Wang, Younan Chen, Lichaun Yang… in Endocrine (2014)

Article

Metabonomics revealed xanthine oxidase-induced oxidative stress and inflammation in the pathogenesis of diabetic nephropathy

Diabetic nephropathy (DN) is a serious complication of diabetes mellitus (DM), which is a major public health problem in the world. To reveal the metabolic changes associated with DN, we analyzed the serum, ur...

**g** Liu, Chengshi Wang, Fang Liu, Yanrong Lu… in Analytical and Bioanalytical Chemistry (2015)

Article

High-fat diet combined with low-dose streptozotocin injections induces metabolic syndrome in Macaca mulatta

Metabolic syndrome (MetS) is associated with abdominal obesity, hyperlipidemia, insulin resistance, and type 2 diabetes mellitus, and increases the risk of cardiovascular disease. Given the complex multifacto...

Linzhao Li, Guangneng Liao, Guang Yang, Yanrong Lu, **aojiong Du, **g** Liu… in Endocrine (2015)

Article

Preparation and characterization of a novel variant of human tumor necrosis factor-related apoptosis-inducing ligand from the rhesus monkey, Macaca mulatta

Human tumor necrosis factor-related apoptosis-inducing ligand (hTRAIL) and its variants are attractive antitumor drug candidates. The predicted amino acid sequence of the functional extracellular domain of Macaca...

Dianlong Jia, Hao Yang, Ze Tao, Lin Wan… in Applied Microbiology and Biotechnology (2016)

Article

Oleic acid protects insulin-secreting INS-1E cells against palmitic acid-induced lipotoxicity along with an amelioration of ER stress

It is demonstrated that unsaturated fatty acids can counteract saturated fatty acids-induced lipotoxicity, but the molecular mechanisms are unclear. In this study, we investigated the protective effects of mon...

**aohong Liu, **n Zeng, Xuanming Chen, Ruixi Luo, Linzhao Li, Chengshi Wang… in Endocrine (2019)

Article

PDGFRβ-targeted TRAIL specifically induces apoptosis of activated hepatic stellate cells and ameliorates liver fibrosis

Liver fibrosis usually progresses to liver cirrhosis and even hepatocellular carcinoma. Since activated hepatic stellate cells (aHSCs) are responsible for liver fibrosis, reducing the quantity of aHSCs was con...

Rui Li, Zhao Li, Yanru Feng, Hao Yang, Qiuxiao Shi, Ze Tao, **gqiu Cheng… in Apoptosis (2020)