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Article
Open AccessAutophagy-deficient macrophages exacerbate cisplatin-induced mitochondrial dysfunction and kidney injury via miR-195a-5p-SIRT3 axis
Macrophages (Mφ) autophagy is a pivotal contributor to inflammation-related diseases. However, the mechanistic details of its direct role in acute kidney injury (AKI) were unclear. Here, we show that Mφ promot...
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Article
Longitudinal model for a dose-finding study for a rare disease treatment
Dose-finding studies in rare diseases are faced with unique challenges including low patient numbers, limited understanding of the dose-exposure-response relationship, variability around the endpoints. In addi...
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Article
Open AccessEfficacy and Safety of Garadacimab in Combination with Standard of Care Treatment in Patients with Severe COVID-19
Garadacimab, a fully human IgG4 monoclonal antibody, inhibits the kallikrein–kinin pathway at a key initiator, activated coagulation factor XII (FXIIa), and may play a protective role in preventing the progres...
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Article
Open AccessCorrection to: Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis
The original article possessed a minor typo in first author, **n Zeng’s name which has since been corrected.
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Article
Open AccessOleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis
Pyroptosis is a novel programmed cell death. It is identified as caspase-1 dependent and characterized by plasma-membrane rupture and release of proinflammatory intracellular contents inculuding IL-1 beta and ...
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Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells
The role of oxidative stress in the pathogenesis of type 2 diabetes (T2D), especially pancreatic β-cell dysfunction and death, has become apparent in the last two decades. Peroxidase- and catalase-based antiox...
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Article
Open AccessIntervention for early diabetic nephropathy by mesenchymal stem cells in a preclinical nonhuman primate model
Diabetic nephropathy (DN) is one of the most severe chronic diabetic complications and the main cause of end-stage renal disease. Chronic inflammation plays a key role in the development of DN. However, few tr...
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Article
Oleic acid protects insulin-secreting INS-1E cells against palmitic acid-induced lipotoxicity along with an amelioration of ER stress
It is demonstrated that unsaturated fatty acids can counteract saturated fatty acids-induced lipotoxicity, but the molecular mechanisms are unclear. In this study, we investigated the protective effects of mon...
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Article
Open AccessProtein–Protein Affinity Determination by Quantitative FRET Quenching
The molecular dissociation constant, Kd, is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions. Recently, we reported the theoretical basis and experimental...
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Article
Open AccessMesenchymal stem cells ameliorate hyperglycemia-induced endothelial injury through modulation of mitophagy
Mitochondrial dysfunction and excessive mitochondrial reactive oxygen species (ROS) are fundamental contributors to endothelial injury in diabetic states. Mesenchymal stem cells (MSCs) have exhibited an extrao...
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Article
Open AccessGLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
Glucagon-like peptide-1 (GLP-1) promotes insulin secretion from pancreatic β-cells in a glucose dependent manner. Several pathways mediate this action by rapid, kinase phosphorylation-dependent, but gene expre...
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Article
High-fat diet combined with low-dose streptozotocin injections induces metabolic syndrome in Macaca mulatta
Metabolic syndrome (MetS) is associated with abdominal obesity, hyperlipidemia, insulin resistance, and type 2 diabetes mellitus, and increases the risk of cardiovascular disease. Given the complex multifacto...
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Article
Assessment of early renal damage in diabetic rhesus monkeys
The objectives of the study were to improve the model system of diabetic nephropathy in nonhuman primates and assess the early renal damage. Diabetes was induced in monkeys by streptozotocin, and the animals ...
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Article
Production and characterization of human soluble CD83 fused with the fragment crystallizable region of human IgG1 in Pichia pastoris
The cell surface protein CD83 belongs to the immunoglobulin superfamily and is highly expressed on mature dendritic cells. The soluble form of CD83, sCD83, is a potential immune suppressor. In a previous study...
