51 Result(s)
within
Protocol
Zheng-**ong **
Life Sciences
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Protocol
Immunohistochemistry and Immunocytochemistry
Immunohistochemistry and immunocytochemistry are powerful techniques for localizing the molecular expression of proteins in tissues and cells, especially when combined with the in situ hybridization technique. Im...
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Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
The ability to measure rare mutational events in mitochondrial genomes from human blood and tissues without resorting to phenotypic selection is invaluable. It is essential for the study of the cause(s) of mitoch...
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Construction of Genomic Libraries in λ-Vectors
Lambda (λ) bacteriophages are viruses that specifically infect bacteria. The genome of λ-phage is a double-stranded DNA molecule approx 50 kb in length (1). In bacterial cells, λ-phage employs one of two pathways...
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Ribozymes as Tools for Suppression of G Protein γ Subunits
RNA suppression approaches provide a rapid survey of gene function. Of these approaches (i.e., antisense oligonucleotides, ribozymes, and RNA interference), ribozymes offer significant advantages by operating ...
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Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems
With the great achievements of recombinant DNA technology, a variety of expression systems, including yeast, baculovirus, adenovirus, and attenuated Salmonella expression systems, have been used for the overex...
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Population and Structure Determination of Hidden Folding Intermediates by Native-State Hydrogen Exchange-Directed Protein Engineering and Nuclear Magnetic Resonance
Structural characterization of folding intermediates has been one of the important steps toward understanding the mechanism of protein folding. However, it has been very difficult to obtain high-resolution str...
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Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
Apoptosis is a genetically controlled process of cell suicide that plays an important role in animal development and in maintaining homeostasis. The nematode Caenorhabditis elegans has proven to be an excellent m...
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Micropropagation of Penthorum chinenseThrough Axillary Buds
Penthorum chinensePursh is a traditional medicinal herb in China. Micropropagation protocol of this plant has been established. The shoot induction rate is high by culturing nodal explants on Murashige and Skoog...
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Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding Interactions
This chapter describes two methods for fabricating microarrays of saccharides for display and interrogation with binding proteins, using fluorescence detection. The first approach is based on the rapid immobil...
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Transplantation of Neural Stem/Progenitor Cells into Develo** and Adult CNS
Neural transplantation has been a long-standing goal for the treatment of neurological injury and disease. The recent discovery of persistent pools of neural stem cells within the adult mammalian brain has rei...
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Computer Modeling of Helicases Using Elastic Network Model
In this chapter, we will demonstrate the usage of a suite of computational techniques based on a coarse-grained elastic network model by applying them to a monomeric helicase—the NS3 helicase of hepatitis C vi...
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Gene Therapy of Salivary Diseases
For many years, our laboratory has been develo** gene transfer approaches for salivary gland disorders that currently lack effective therapy. The purpose of this chapter is to describe key methods used in th...
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Real-Time Measurement of Murine Hippocampus NO Levels in Response to Cerebral Ischemia/Reperfusion
Nitric oxide has been implicated as a mediator of synaptic transmission and a pathological factor in stroke/reperfusion. The purpose of this study was to detect the change of nitric oxide concentration in rat ...
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Protocol
Stochastic Analysis of Gene Expression
In this chapter, stochasticity in gene expression is investigated using \( \Omega \) -expansion technique. Two theoretical mod...
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Lipases as Biocatalysts for the Synthesis of Structured Lipids
Structured lipids (SL) are broadly referred to as modified or synthetic oils and fats or lipids with functional or pharmaceutical applications. Some structured lipids, such as triglycerides that contain both l...
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Culture of Choroid Plexus Epithelial Cells and In Vitro Model of Blood–CSF Barrier
Chemical homeostasis in the extracellular fluid of the central nervous system (CNS) is maintained by two brain barrier systems, i.e., the blood–brain barrier (BBB) that separates the blood circulation from bra...
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Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
The nematode Caenorhabditis elegans is an excellent model organism for studying the mechanisms controlling cell death, including apoptosis, a cell suicide event, and necrosis, pathological cell deaths caused by ...
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Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins
Principles underlying the recording of high-quality/resolution images of two-dimensional crystals of membrane proteins are discussed in the context of instrumental conditions and operational procedures. A deta...
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Metabolic Characterization of Hematopoietic Stem Cells
An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSC...
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Analysis of Protein Conformational Transitions Using Elastic Network Model
In this chapter, we demonstrate the usage of a coarse-grained elastic network model to analyze protein conformational transitions in the NS3 helicase (NS3hel) of Hepatitis C virus (HCV). This analysis allows u...
