4 Result(s)
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Protocol
Vanesa Robles
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Protocol
In Vitro Induction of Teleost PGCs
are unique cells in an . These cells contain all genetic information and therefore represent the best source to store maternal and paternal genomes until is achieved. However, the number of these cells i...
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Protocol
Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa
Reactive oxygen species (ROS) could have a negative impact on sperm cellular function and viability. This chapter describes a protocol for oxidative stress evaluation using dichlorofluorescein (DCF) which can ...
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Protocol
Chapter 19 Cryopreservation Effect on Genetic Function: Neonatal Outcomes
Cryopreservation is a well-established technique commonly used in clinical practice. It is used widely for the conservation of gametes and embryos that will be used later for insemination or in vitro fertiliza...
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Protocol
Flow Cytometric Methods for Sperm Assessment
Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for evaluating critical aspects of sperm quality: the double stain SYB...
