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    Protocol

    PAM-Less CRISPR-SpRY Genome Editing in Plants

    Engineered SpCas9 variant, SpRY, has been demonstrated to facilitate protospacer adjacent motif (PAM) unrestricted targeting of genomic DNA in various biological systems. Here we describe fast, efficient, and ...

    Simon Sretenovic, Xu Tang, Qiurong Ren, Yong Zhang, Yi** Qi in Plant Genome Engineering (2023)

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    Protocol

    Base Editing in Poplar Through an Agrobacterium-Mediated Transformation Method

    CRISPR-Cas9 systems have revolutionized genome editing in plants and facilitated gene knockout and functional genomic studies in woody plants, like poplar. However, in tree species, previous studies have only ...

    Gen Li, Simon Sretenovic, Gary Coleman, Yi** Qi in Plant Genome Engineering (2023)