16 Result(s)
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Protocol
Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
Sequences with the potential to form intramolecular G-quadruplexes (G4-structures) are found in highly nonrandom distributions in the genomes of diverse organisms. These sequences are associated with nucleic a...
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Protocol
High-Throughput SNP Detection Based on PCR Amplification on Magnetic Nanoparticles Using Dual-Color Hybridization
A microarray-based method for detecting single nucleotide polymorphisms (SNPs) using solid-phase polymerase chain reaction (PCR) on magnetic nanoparticles (MNPs) was developed. In this method one primer with a...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Protocol
Size Fractionation of Cell-Free DNA in Maternal Plasma and Its Application in Noninvasive Detection of Fetal Single Gene Point Mutations
Recent studies have shown that cell-free fetal DNA in maternal plasma can be enriched by means of size fractionation. This technique makes use of the smaller size of fetal DNA fragments compared with maternal ...
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Protocol
Clustering Analysis of SAGE Transcription Profiles Using a Poisson Approach
To gain insights into the biological function and relevance of genes using serial analysis of gene expression (SAGE) transcription profiles, one essential method is to perform clustering analysis on genes. A s...
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Protocol
Application of siRNA Against SARS in the Rhesus Macaque Model
Containment of the SARS coronavirus (SCV) outbreak was accompanied by the rapid characterization of this new pathogen's genome sequence in 2003, encouraging the development of anti-SCV therapeutics using short...
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Protocol
Real-Time Polymerase Chain Reaction Gene Expression Assays in Biomarker Discovery and Validation
Biomarkers have shown great potential in molecular classification and targeted treatment of human diseases. Biomarkers are also expected to play an increasingly important role in all phases of drug development...
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Protocol
High-Throughput Genoty** of Single Nucleotide Polymorphisms with High Sensitivity
The ability to analyze a large number of genetic markers consisting of single nucleotide polymorphisms (SNPs) may bring about significant advance in understanding human biology. Recent development of several h...
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Protocol
Protein Chip for Detection of DNA Mutations
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...
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Protocol
Designing PCR Primer for DNA Methylation Map**
DNA methylation is an epigenetic mechanism of gene regulation, and aberrant methylation has been associated with various types of diseases, especially cancers. Detection of DNA methylation has thus become an i...
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Protocol
In Silico Prediction of Peptide-MHC Binding Affinity Using SVRMHC
The binding between peptide epitopes and major histocompatibility complex (MHC) proteins is a major event in the cellular immune response. Accurate prediction of the binding between short peptides and class I ...
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Protocol
Family-Based Linkage Disequilibrium Tests Using General Pedigrees
Linkage disequilibrium (LD) map** has been established as a promising approach to identifying disease genes. The presence of a disease gene located near a marker locus may cause LD between the marker and the...
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Protocol
Nanotechnology
Microarrays are important tools for high-throughput analysis of biomolecules. The use of microarrays for parallel screening of nucleic acid and protein profiles has become an industry standard. A few limitatio...
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Protocol
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy
Human telomerase reverse transcriptase (hTERT) represents a universal tumor-associated antigen to activate specific immune response in cancer immune therapy. Peptides derived from hTERT are presented by major ...
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Protocol
Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference
More than 85% of human cancers and over 70% of immortalized human cell lines have highly elevated telomerase activity. In contrast, telomerase activity is down-regulated in most human adult somatic cells, exce...
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Protocol
Inhibition of Telomerase by Targeting MAP Kinase Signaling
Constitutive activation of the mitogen-activated protein (MAP) kinase signaling pathway by oncogenic stimulation is widespread in human cancers. With the recently demonstrated links between MAP kinase, histone...
