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  1. No Access

    Protocol

    Methods for TALEN-Mediated Genomic Manipulations in Drosophila

    TALEN (transcription activator-like effector nuclease) is a powerful tool for gene disruption and other genomic modifications. In the past 3 years or so, it has attracted eyes from every corner of the biologic...

    Jiyong Liu, Yawen Guo, Changqing Li, Yixu Chen, Renjie Jiao in TALENs (2016)

  2. No Access

    Protocol

    Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos

    The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...

    You E. Li, Bryan G. Allen, Daniel L. Weeks in Xenopus Protocols (2012)