15 Result(s)
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Protocol
High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry Method for the Identification and Quantification of Lipids in Liposomes
Liposomes are spherical, closed vesicles consisting of at least one lipid bilayer with a water chamber and are widely used to encapsulate bioactive molecules. Lipid membranes, composed of different types of li...
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Protocol
Transwell In Vitro Cell Migration and Invasion Assays
Cell migration and invasion have essential roles in both normal physiology and disease. As such, methodologies to assess cell migratory and invasive capacities are necessary to elucidate normal cell processes ...
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Protocol
Preparation and Characterization of Trastuzumab Fab-Conjugated Liposomes (Immunoliposomes)
Immunoliposomes are made by conjugating antibodies or antibody fragments on liposome surfaces. Antibody fragments Fab’, single-chain Fv fragments (scFv), or new constructs such as nanobodies are commonly used ...
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Protocol
Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effectiv...
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Protocol
Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip
Reconstituted model membrane systems are powerful platforms to tackle interesting problems existing in membrane biology. One of the barriers to efficient drug delivery, as therapeutics to disease, is the physi...
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Protocol
Reconstitution of Morphogen Signaling Gradients in Cultured Cells
Development of multicellular organisms depends on the proper establishment of signaling information in space and time. Secreted molecules called morphogens form concentration gradients in space and provide pos...
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Protocol
A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore
Many enzymatic activity assays are based on either (1) identifying and quantifying the enzyme with methods such as western blot or enzyme-linked substrate assay (ELISA) or (2) quantifying the enzymatic reactio...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Protocol
Delivery of DNA into Tumors
Delivery of plasmid DNA encoding therapeutic genes into tumors is one of the main applications of electroporation. This chapter summarizes various investigators' electroporation parameters for intratumoral gen...
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Protocol
Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation
Interleukin 12 (IL-12) is effective in treating systemic microscopic malignancies by inducing T helper 1 (TH1) response, inhibiting angiogenesis, and triggering secondary cytokine production. Unfortunately, daily...
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Protocol
Periodic Assembly of Nanospecies on Repetitive DNA Sequences Generated on Gold Nanoparticles by Rolling Circle Amplification
Periodical assembly of nanospecies is desirable for the construction of nanodevices. We provide a protocol for the preparation of a gold nanoparticle (AuNP)/DNA scaffold on which nanospecies can be assembled i...
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Protocol
Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs
Bleomycin and Interleukin 12 have been used clinically to treat tumors; however, the co-administration of Bleomycin and Interleukin 12 followed by electroporation has not been tested clinically. In this study,...
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Protocol
Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination
Cyclophosphamide and Interleukin-12 (IL-12) have been successfully used in clinical trials for treating malignancies. In this study, we explore the coadministration of cyclophosphamide and IL-12 plasmid DNA fo...
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Protocol
Protein Chip for Detection of DNA Mutations
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...
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Protocol
Nanotechnology
Microarrays are important tools for high-throughput analysis of biomolecules. The use of microarrays for parallel screening of nucleic acid and protein profiles has become an industry standard. A few limitatio...
