6 Result(s)
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English
Biomedicine
Animal Genetics and Genomics
Human Genetics
Lu**g Li
Protocol
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Protocol
Methods for TALEN-Mediated Genomic Manipulations in Drosophila
TALEN (transcription activator-like effector nuclease) is a powerful tool for gene disruption and other genomic modifications. In the past 3 years or so, it has attracted eyes from every corner of the biologic...
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Protocol
Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...
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Protocol
Directed Evolution of Adeno-Associated Virus (AAV) as Vector for Muscle Gene Therapy
Adeno-associated virus (AAV) is emerging as a vector of choice for muscle gene therapy because of its effective and stable transduction in striated muscles. AAV naturally evolve into multiple serotypes with di...
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Protocol
Monitoring Murine Skeletal Muscle Function for Muscle Gene Therapy
The primary function of skeletal muscle is to generate force. Muscle force production is compromised in various forms of acquired and/or inherited muscle diseases. An important goal of muscle gene therapy is t...
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Protocol
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
Lentiviral vectors are able to transduce non-dividing cells and maintain sustained long-term expression of the transgenes. Many cell types including brain, liver, muscle, and hematopoietic stem cells have been...
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Protocol
Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy
The development of trans-splicing vectors opens the door for delivering a large therapeutic gene with adeno-associated viral vectors (AAV). One potential application is to deliver the 6 kb mini-dystrophin gene fo...
