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    Protocol

    In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency

    The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) system has become a popular toolkit for editing genomes of interest in a wide variety of organisms. The Ca...

    Subhasis Karmakar, Deeptirekha Behera, Mirza Jaynul Baig… in CRISPR-Cas Methods (2021)

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    Protocol

    CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome

    Base editing is a recently developed technology that enables us to install point mutation in the genome precisely. The fusion of nucleotide deaminase with Cas9 nickase gives rise to base editors. Like the conv...

    Kutubuddin A. Molla, Yinong Yang in CRISPR-Cas Methods (2020)

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    Protocol

    Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine

    The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has revolutionized our ability to manipulate DNA...

    Kutubuddin A. Molla, Subhasis Karmakar, M. Tofazzal Islam in CRISPR-Cas Methods (2020)