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    Protocol

    Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos

    The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...

    You E. Li, Bryan G. Allen, Daniel L. Weeks in Xenopus Protocols (2012)

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    Protocol

    Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

    Bacteriophage φC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integras...

    Bryan G. Allen, Daniel L. Weeks in Microinjection (2009)