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    Protocol

    Luminex-Coupled EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Antigen-Specific T cells in Human Peripheral Blood

    We describe a Luminex-coupled EliFACS assay that integrates multiplexing technology, enzyme-linked immunospot (ELISPOT), and intracellular cytokine FACS staining for the detection of multiple parameters of ant...

    HuiJuan Lv, Khadir Raddassi, Myra A. Lipes in Immunological Tolerance (2019)

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    Protocol

    High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

    The secretory pathway of the yeast Pichia pastoris has been engineered to produce complex human-type N-glycans (Choi et al., Proc Natl Acad Sci USA 100:5022–5027, 2003; Hamilton et al., Science 301:1244–1246, 200...

    Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown… in Therapeutic Proteins (2012)