26 Result(s)
within
Cell Biology
**an Qi
Protocol
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Protocol
Expression of Heterologous Integrin Genes
Cell-extracellular matrix interactions are mediated predominantly by integrins, cell-surface receptors that exist as heterodimers of noncovalently associated α and β subunits (1). Integrins have been implicated i...
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Functional Analysis of Cytoskeletal Components in the Develo** Visual System of Drosophila
A central issue in neuroscience is to understand the mechanisms underlining the stereotyped patterns of neuronal connectivity. Studies of neuronal development in both vertebrates and invertebrates have establi...
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Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential sign...
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μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
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Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS
Grafting neural stem cells is a widely used experimental approach to central nervous system (CNS) repair after trauma or neurodegeneration. It is likely to be a realistic clinical therapy for human CNS disorde...
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Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the ...
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Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Using Drosophila actin-binding protein Dunc-115 as an example, this chapter describes an MARCM based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise des...
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Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors
Amplification of the gene encoding the epidermal growth factor receptor (EGFR) occurs commonly in glioblastoma (GBM), leading to activation of downstream kinases, including phosphatidylinositol 3′-kinase (PI3K...
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Assessing Reprogramming by Chimera Formation and Tetraploid Complementation
Pluripotent stem cells can be evaluated by pluripotent markers expression, embryoid body aggregation, teratoma formation, chimera contribution and even more, tetraploid complementation. Whether iPS cells in ge...
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A Chimeric Embryoid Body Model to Study Vascular Morphogenesis
Embryonic stem cell (ESC)-derived embryoid body (EB) is a unique model for studying vascular development, in that it provides a three-dimensional microenvironment that mimics an in vivo milieu. When using gene...
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Membrane Nanotube Formation in Osteoclastogenesis
Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastoge...
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Monitoring Wnt/β-Catenin Signaling in Skin
Wnt signaling through β-catenin plays a crucial role in skin development and homeostasis. Disruption or hyperactivation of this pathway results in skin defects and diseases (Lim and Nusse, Cold Spring Harb Per...
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Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functi...
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Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes
Sex chromosomes and sex-linked genes usually show unusual features comparing to the rest of the genome and thus are of particular interests to evolutionary and developmental biologists. Here we describe recent...
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Probing the Role of Cyclic di-GMP Signaling Systems in Disease Using Chinese Radish
The determination of the genome sequences of pathogenic bacteria has facilitated functional analyses that aim to understand the molecular basis of virulence. In particular, genome sequence information of the p...
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Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet
Split thickness skin grafts (STSGs) are one of the standard treatments available for full thickness wound repair when full thickness grafts (FTGs) are not viable, such as in the case of wounds with large surfa...
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Efficient Culture of Intestinal Organoids with Blebbistatin
The intestinal epithelium is one of the most rapidly self-renewing tissues throughout life in mammals. A small population of stem cells at the base of crypt in the epithelium can continually self-renew and giv...
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Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis
Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitr...
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A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
Extracellular vesicles (EVs) are membrane-enclosed vesicles released by cells. They carry proteins, nucleic acids, and metabolites which can be transferred to a recipient cell, locally or at a distance, to eli...
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A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1
In conditions of cellular stress and nutrient shortage, macroautophagy (hereafter referred to as autophagy) assures the degradation of dysfunctional macromolecules and organelles as it liberates energy resourc...
