44 Result(s)
within
Protocol
**an Qi
Biomedicine
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Protocol
Photoacoustic Lifetime Imaging of Hypoxia
We present an approach based on photoacoustic lifetime imaging (PALI) to map the distribution of oxygen partial pressure (pO2) in tissue. This method utilizes methylene blue, a dye widely used in clinical applica...
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A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy
Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone...
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Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy
Retinal neovascularization is one of the leading causes of vision loss and a hallmark of proliferative diabetic retinopathy (PDR). The immune system is observed to be involved in the pathogenesis of diabetic r...
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Electrogenerated Chemiluminescence Method for Determination of 5-Hydroxymethylcytosine in DNA
5-methylcytosine (5-mC) is regarded as the fifth base of DNA, while 5-hydroxymethylcytosine (5-hmC) is regarded as the sixth base of DNA. With the catalysis of ten–eleven translocation proteins, 5-mC can be co...
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A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1
In conditions of cellular stress and nutrient shortage, macroautophagy (hereafter referred to as autophagy) assures the degradation of dysfunctional macromolecules and organelles as it liberates energy resourc...
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In Vivo Migration and Tfh Cell Interactions
Follicular T-helper cells play a key role in orchestrating the germinal center response. Cellular dynamics revealed by intravital imaging have helped deepen our understanding of how follicular T-helper cells d...
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Detection of 5-Formylcytosine and 5-Formyluracil Based on Photo-Assisted Domino Reaction
5-Formylcytosine (5fC) is a recently identified DNA modification in mammalian genome, derived from 5-methylcytosine (5mC) by TET-mediated oxidation. It has been demonstrated to be a critical intermediate durin...
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Tetramer-Associated T Cell Receptor Sequencing
Linking antigen specificity to T cell receptor (TCR) sequences is critical, albeit challenging, to both understanding T cell biology and develo** T cell-based therapeutics. Here, we describe in detail tetram...
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Discovery of Covalent Drugs Targeting the Key Enzymes of SARS-CoV-2 Using SCARdock
Historically, covalent drugs were avoided in drug development process due to the possible toxicity linked to the covalent binding of such drugs and cellular proteins. However, recent years have witnessed the f...
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Protocol
Assembly of TALEN and mTALE-Act for Plant Genome Engineering
Transcription activator-like effector (TALE) is a DNA-binding domain that can be paired with a nuclease to create DNA double-strand breaks, or with an effector protein to alter gene transcription. The ability ...
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Precision Medicine in Tissue Engineering on Bone
With the rapidly development of clinical treatments, precision medicine has come to people eyes with the requirement according to different people and different disease situation. So precision medicine is call...
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Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus
Interleukin (IL)-22 belongs to the IL-10 family of cytokines. IL-22 exerts its biological effects via members of the cytokine receptor family class 2. CD4+ T helper (Th) cells predominantly producing IL-22 have b...
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Methods for Metabolic Engineering of a Filamentous Trichoderma reesei
In this work, we describe genetic tools and techniques for engineering Trichoderma reesei for the production of farnesene. Enhanced production of farnesene was used as an example of this methodology; as were the ...
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Application of Deep Mutational Scanning in Hepatitis C Virus
Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-...
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In Vivo Pharmacology Models for Cancer Target Research
Experimental animal tumor models have been broadly used to evaluate anticancer drugs in the preclinical setting. They have also been widely applied for drug target discovery and validation, which usually follo...
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Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes
Drug-induced cholestasis poses a major hurdle for the pharmaceutical industry as it is one the primary mechanisms of drug-induced liver injury. Hence, detection of drug-induced cholestasis during the early sta...
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Protocol
Genetic Studies of Tic Disorders and Tourette Syndrome
Tourette syndrome (TS) is a complex disorder characterized by repetitive, sudden, and involuntary movements or vocalizations, called tics. Tics usually appear in childhood, and their severity varies over time....
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Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input
DNA methylation changes are dynamic processes which occur at cytosines of CpG dinucleotides and contribute to normal development but also to diseases. DNA methylation changes are most effective in promoters an...
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Protocol
Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function
Transporters play a crucial role in the uptake of endo- and exogenous molecules in hepatocytes and efflux into the bile. The bile salt export pump (BSEP; ABCB11) is of major importance for efflux of bile salts to...
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Novel Methods for Family-Based Genetic Studies
The recent development of microarray and sequencing technology allows identification of disease susceptibility genes. Although the genome-wide association studies (GWAS) have successfully identified many genet...
