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A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy

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Chemokine-Glycosaminoglycan Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2597))

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Abstract

Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone many changes to reduce the stress response on the animals and improve the reliability of the data. Here, we detail our method of performing hindlimb suspension in mice that minimizes stress, maximizes the replicability of the data, and uses space efficiently.

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Acknowledgments

This study was supported by grants from National Institutes of Health to HZ (HL153876 and AR067766).

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Authors and Affiliations

  1. Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, USA

    Laura Kutz & Hua Zhu

  2. Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, USA

    Tong Zhou

  3. Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, USA

    Qi Chen

Authors
  1. Laura Kutz
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  2. Tong Zhou
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  3. Qi Chen
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  4. Hua Zhu
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Corresponding author

Correspondence to Hua Zhu .

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Editors and Affiliations

  1. Biodesign Institute, Arizona State University, Tempe, AZ, USA

    Alexandra R. Lucas

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kutz, L., Zhou, T., Chen, Q., Zhu, H. (2023). A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy. In: Lucas, A.R. (eds) Chemokine-Glycosaminoglycan Interactions. Methods in Molecular Biology, vol 2597. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2835-5_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2835-5_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2834-8

  • Online ISBN: 978-1-0716-2835-5

  • eBook Packages: Springer Protocols

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