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Article
Enrichment of Functional Characteristics in the Okara by the Fermentation of Rhizopus azygosporus
Okara is a solid waste of soybean-processed foods; it is normally used as a low-priced product, such as fertiliser and feed. An alternative method for the convertion of okara to valorized products is required.
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Article
Open AccessNovel purification method and antibiotic activity of recombinant Momordica charantia MAP30
Ribosome-inactivating proteins (RIPs) are a group of enzymes originally isolated from plants that possess the ability to damage ribosomes in an irreversible manner, leading to inhibition of protein synthesis i...
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Article
Open AccessRhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis
Autophagy is an essential process in the control of cellular homeostasis. It enables cells under certain stress conditions to survive by removing toxic cellular components, and may protect cells from apoptosis...
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Article
Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum
In this study the mechanism of avian reovirus (ARV) S1133-induced pathogenesis was investigated, with a focus on the contribution of ER stress to apoptosis. Our results showed that upregulation of the ER stres...
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Article
Open AccessA highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
Cellulose, which is the most abundant renewable biomass on earth, is a potential bio-resource of alternative energy. The hydrolysis of plant polysaccharides is catalyzed by microbial cellulases, including endo...
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Article
Open AccessFunctional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Neocallimastix patriciarum is one of the common anaerobic fungi in the digestive tracts of ruminants that can actively digest cellulosic materials, and its cellulases have great potential for hydrolyzing cellulos...
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Article
The identification, purification, and characterization of STXF10 expressed in Streptomyces thermonitrificans NTU-88
Multiple xylanolytic enzymes of Streptomyces thermonitrificans NTU-88 were induced by oat-spelt xylan and separated by two-dimensional polyacrylamide and zymogram gels. Nineteen clear spots differed in pI and mol...
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Article
Characterization of the activities of p21Cip1/Waf1 promoter-driven reporter systems during camptothecin-induced senescence-like state of BHK-21 cells
It was attempted in this work to establish a cell line in which senescent cells can be readily and directly identified in situ in live culture. Transcriptional activation of p21Cip1/Waf1 gene is known to be one o...
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Article
Immunogold labeling of CM-cellulase in Sinorhizobium fredii CCRC15769 by electron microscopy
The symbiotic roles of rhizobia with legumes have been extensively made use of in the agricultural field. An unresolved question about the infection process is the nature of the Rhizobium enzymes that degrade the...