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Article
Melanopsin signalling in mammalian iris and retina
Non-mammalian vertebrates have an intrinsically photosensitive iris and thus a local pupillary light reflex (PLR). In contrast, it is thought that the PLR in mammals generally requires neuronal circuitry conne...
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Article
Characterisation of explanted endothelial cells from mouse aorta: electrophysiology and Ca2+ signalling
We describe here the isolation and primary culture of endothelial cells from mouse aorta ("primary explant technique"). These cells provide an excellent model for functional studies in transgenic mice. The pr...
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Article
Injection of catalytic subunit of cAMP-dependent protein kinase into isolated cardiac myocytes
Cyclic adenosine 3′,5′-monophosphate (cAMP) or the free catalytic subunit (C) of the cAMP-dependent protein kinase were pressure injected into single guinea pig ventricular cells. The following results were ob...
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Article
Injection of subunits of cyclic AMP-dependent protein kinase into cardiac myocytes modulates Ca2+ current
β-Adrenergic stimulation of the heart is thought to increase cardiac muscle contractility by activation of cyclic AMP-dependent protein kinase and concomitant increase in the phosphorylation of certain proteins.....
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Article
Injection of isolated subunits of cAMP-dependent protein kinase II into single adult cardiac myocytes modulates the slow Ca++ inward current