26 Result(s)
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Article
Masahiro Sugiura
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
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Article
Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1
We isolated a 38 kDa ssDNA-binding protein from the unicellular cyanobacterium Synechococcus sp. strain PCC 6301 and determined its N-terminal amino acid sequence. A genomic clone encoding the 38 kDa protein was ...
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Characterization of a cDNA encoding a novel type of RNA-binding protein in tobacco: its expression and nucleic acid-binding properties
A cDNA encoding an RNA-binding protein (ribonucleoprotein or RNP) was isolated from a tobacco (Nicotiana sylvestris) cDNA library. The predicted protein (termed RGP-2) is 259 amino acids in length and consists of...
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Structure and expression of the tobacco nuclear gene encoding the 33 kDa chloroplast ribonucleoprotein
Tobacco chloroplasts contain a family of ribonucleoproteins (RNPs) which appear to be involved in mRNA processing and splicing in chloroplasts. We have characterized a new cDNA, 33k-6, potentially encoding a t...
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Active transcription from a promoter positioned within the coding region of a divergently oriented gene: the tobacco chloroplastrp132 gene
A new transcription unit has been identified and characterized in the small single-copy region of tobacco chloroplast DNA. A primary transcript (1550 nucleotides) spanning the entire transcription unit contain...
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Chloroplast DNA of black pine retains a residual inverted repeat lacking rRNA genes: nucleotide sequences of trnQ, trnK, psbA, trnI and trnH and the absence of rps16
A physical map of black pine (Pinus thunbergii) chloroplast DNA (120 kb) was constructed and two separate portions of its nucleotide sequence were determined. One portion contains trnQ-UUG, ORF510, ORF83, trnK-UU...
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Rice chloroplast RNA polymerase genes: The absence of an intron in rpoC1 and the presence of an extra sequence in rpoC2
The chloroplast genome contains sequences homologous to the Escherichia coli rpoA, rpoB and rpoC genes. The Choroplast rpoC gene is divided into rpoC1 and rpoC2, of which rpoC1 contains an intron. Comparison of t...
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The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals
The entire chloroplast genome of the monocot rice (Oryza sativa) has been sequenced and comprises 134525 bp. Predicted genes have been identified along with open reading frames (ORFs) conserved between rice and t...
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Genes for the ribosomal proteins S12 and S7 and elongation factors EF-G and EF-Tu of the cyanobacterium, Anacystis nidulans: Structural homology between 16S rRNA and S7 mRNA
A 6.5 kb region from the genome of the cyanobacterium, Anacystis nidulans 6301 was cloned using the tobacco chloroplast gene for ribosomal protein S12 as a probe. Sequence analysis revealed the presence of genes ...
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Six chloroplast genes (ndhA-F) homologous to human mitochondrial genes encoding components of the respiratory chain NADH dehydrogenase are actively expressed: Determination of the splice sites in ndhA and ndhB pre-mRNAs
Sequences (ndhA-F) homologous to human mitochondrial genes for components of the respiratory chain NADH dehydrogenase have been found in the chloroplast DnA of tobacco. The ndhA, D, E and F sequences correspondin...
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Rapid splicing and stepwise processing of a transcript from the psbB operon in tobacco chloroplasts: Determination of the intron sites in petB and petD
Expression of the psbB gene cluster in tobacco chloroplasts has been studied. This cluster contains the genes for the 51 kDa chlorophyll a apoprotein (psbB) and the 10 kDa phosphoprotein (psbH) of photosystem II,...
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Analysis of the promoter region in the rrnA operon from a blue-green alga, Anacystis nidulans 6301
The nucleotide sequence has been determined of a 1431 base pair (bp) region upstream from the 16S rRNA gene in the rrnA operon of a blue-green alga, Anacystis nidulans 6301. The transcriptional initiation site ha...
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Intron in the gene for the ribosomal protein S16 of tobacco chloroplast and its conserved boundary sequences
The nucleotide sequence of a tobacco chloroplast gene coding for a protein homologous to Escherichia coli ribosomal protein S16 (rps16) has been determined. It is located 690 bp upstream from the gene for tRNALys
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Article
Genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase constitute a single operon in a cyanobacterium Anacystis nidulans 6301
The structure and transcription of the genes for the large (LS) and small subunits (SS) of ribulose-1,5-bisphosphate carboxylase/oxygenase of Anacystis nidulans 6301 were studied. The SS gene is located 93 base p...
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Article
Gene organization of chloroplast DNA from the broad bean Vicia faba
Six protein genes have been mapped on broad bean chloroplast DNA by Southern hybridization using the tobacco chloroplast genes as probes. In broad bean chloroplast DNA, the genes for the β and ε subunits of pr...
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Location and nucleotide sequence of a tobacco chlorophlast DNA segment capable of replication in yeast
A 1.7 Kbp EcoRI fragment of Nicotiana tabacum chloroplast DNA cloned in YIp5, consisting of pBR322 and the yeast ura3 gene, possessed ars (autonomously replicating sequences) activity in Saccharomyces cerevisiae....
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Article
Nucleotide sequence and transcription of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum
The nucleotide sequence of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum has been determined. This gene is located 535 base pairs (bp) away from the inverted repeat on the right...
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Sequence analysis of the junctions between a large inverted repeat and single-copy regions in tobacco chloroplast DNA
Tobacco chloroplast DNA contains a large inverted repeat sequence of 26 kilobase pairs (kbp). The inverted repeat is separated by 20 kbp small single-copy and 90 kbp large single-copy regions. We have cloned f...
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Nucleotide sequence of the 16S-23S spacer region in the rrnA operon from a blue-green alga, Anacystis nidulans
The nucleotide sequence of an entire spacer region between the 16S and 23S rRNA genes of the rrnA operon from a blue-green alga, Anacystis nidulans, has been determined. The spacer region is 545 base pairs long a...
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Article
Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast
An autonomously replicating sequence (ars) of Nicotiana tabacum was cloned into the EcoRI site of YIp5, which consists of pBR322 and the yeast ura3 gene. Recombinant DNA was capable of transforming ura3- Saccarom...
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Article
The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans
The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans, has been determined. Its coding region is estimated to be 1,487 base pairs long, which is nearly identical ...
