16 Result(s)
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Article
Characterization of 5′-proximal sequence of mouse GABA transporter gene (GAT-1)
The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 p...
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Article
Analysis of the 5' flanking sequence of the human norepinephrine transporter gene
The human norepinephrine transporter(NET) gene was cloned and structurally analyzed. The far 5' fragment containing exon 1 (a non-coding exon) and exon 2 was sequenced. The transcription start site of the gene...
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Article
Val 70, Phe 72 and the last seven amino acid residues of C-terminal are essential to the function of norepinephrine transporter
The norepinephrine transporter(NET) is a member of the Na+/Cl- dependent neurotransmitter transporter family and constitutes the target of several clinically important antidepressants. To delineate the critical a...
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Article
γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology
γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric aci...
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Article
Transgenic mice overexpressing γ-aminobutyric acid transporter subtype I develop obesity
Transgenic mice ubiquitously overexpressing murine r-aminobutyric acid transporter subtype I were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased ...
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Article
Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to kainic acid-induced seizure in transgenic mice
γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiqui.....
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Article
Adenovirus-mediated expression of pig α(1, 3) galactosyltransferase reconstructs Gal a(1, 3) Gal epitope on the surface of human tumor cells
Gal α(1, 3) Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by α(1, 3) galactosyltransferase [α(1, 3) GT] enzyme on the cells of lower mammalian animals such as pigs and mice. Human ha...
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Article
GABA transporter 1 transcriptional starting site exhibiting tissue specific difference
GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To ad...
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Article
A mRNA molecule encoding truncated excitatory amino acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event
Glutamate transporter EAAC1 removes excitatory neurotransmitter in central nervous system, and also absorbs glutamate in epithelia of intestine, kidney, liver and heart for normal cell growth. When a mouse cDN...
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Article
Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand
It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpre...
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Article
Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I (GAT1)
It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABAA and GABAB receptors were present in testis and sperm, and might mediate the acrosom...
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Article
Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons
To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro.
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Article
A20 overexpression under control of mouse osteocalcin promoter in MC3T3-E1 cells inhibited tumor necrosis factor-alpha-induced apoptosis
To construct an A20 expression vector under the control of mouse osteocalcin promoter (OC-A20), and investigate osteoblastic MC3T3-E1 cell line, which stably overexpresses A20 protein prevented tumor necrosis ...
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Article
Cynomorium songaricum Extracts Functionally Modulate Transporters of γ-Aminobutyric Acid and Monoamine
Cynomorium songaricum Rupr. (SY) is a central nervous system-oriented herb material that has actions of anti-dementia, anti-epilepsy, and anti-stress. It is unclear whether SY would be biologically active in func...
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Article
Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation
Human amniotic epithelial cells (hAECs), derived from the innermost layer of the term placenta closest to the fetus, have been shown to be potential seed cells for allogeneic cell therapy. Previous studies hav...
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Article
Human amniotic epithelial stem cell is a cell therapy candidate for preventing acute graft-versus-host disease
Graft-versus-host disease (GVHD), an immunological disorder that arises from donor T cell activation through recognition of host alloantigens, is the major limitation in the application of allogeneic hematopoi...
