16 Result(s)
within
Article
J. Hescheler
Cell Biology
Molecular Medicine
Receptors
-
Article
Injection of isolated subunits of cAMP-dependent protein kinase II into single adult cardiac myocytes modulates the slow Ca++ inward current
-
Article
Does the organic calcium channel blocker D600 act from inside or outside on the cardiac cell membrane?
The effects of extra- and intracellularly applied D600 (methoxyverapamil) and D890 (a quarternary derivative) on the action potentials of isolated guinea pig myocytes were compared. We also studied the extrace...
-
Article
Inward-rectifying channels in isolated patches of the heart cell membrane: ATP-dependence and comparison with cell-attached patches
Inward rectifying potassium single-channel currents were studied in the membrane of guinea pig cardiac myocytes. In isolated inside-out patches two different channels were observed: a channel of 25 pS conducta...
-
Article
Modulation of Ca current during the phosphorylation cycle in the guinea pig heart
The calcium current (I Ca) in the heart is increased by phosphorylation of a protein which is part of, or close to, the Ca channel. The phosphorylation is catalysed by cAMP-dependent protein kinase (cAMP-PK). The...
-
Article
On the mechanism of muscarinic inhibition of the cardiac Ca current
The mechanism of muscarinic inhibition of the Ca-current (I Ca) was studied in ventricular myocytes of guinea pig hearts and the following results were obtained. 1. Acetylcholine (ACh) in concentrations up to 10−...
-
Article
The protein-specific phosphatase 1 antagonizes the β-adrenergic increase of the cardiac Ca current
In isolated ventricular cells from the adult guinea pig heart the slow Ca current was recorded during β-adrenergic stimulation and during cell dialysis with a protein-specific phosphatase-1 (PPase-1).
-
Article
Changes of membrane currents in cardiac cells induced by long whole-cell recordings and tolbutamide
Single isolated myocytes were obtained from the ventricles of adult guinea pig hearts. The whole-cell recording configuration of the patch-clamp technique was used to measure membrane currents. A decrease (run...
-
Article
On the mechanism of histamine induced enhancement of the cardiac Ca2+ current
In guinea pig ventricular myocytes, the effect of histamine on the slow Ca2+ current (ICa) was studied and the following results were obtained: (1) Superfusion of cells with histamine resulted in a dose-dependent...
-
Article
“Run-down” of the Ca current during long whole-cell recordings in guinea pig heart cells: role of phosphorylation and intracellular calcium
We examined by a statistical approach the decrease of the Ca current (“run-down”) during long-lasting recordings with the whole-cell patch-clamp technique in guinea pig ventricular myocytes. The results are as...
-
Article
Effects of a protein phosphatase inhibitor, okadaic acid, on membrane currents of isolated guinea-pig cardiac myocytes
The effects of a protein phosphatase inhibitor, okadaic acid (OA), were studied on membrane currents of isolated myocytes from guinea-pig cardiac ventricle. The whole-cell Ca2+ current (I Ca) was recorded as peak...
-
Article
A possible physiological role of the Ca-dependent protease calpain and its inhibitor calpastatin on the Ca current in guinea pig myocytes
The decrease (‘run-down’) of the L-type Ca2+-current during long-lasting recordings with the whole-cell patch-clamp technique was examined in guinea pig ventricular myocytes. We have tested whether proteolysis is...
-
Article
Calcium currents of neuroblastoma × glioma hybrid cells after cultivation with dibutyryl cyclic AMP and nickel
The long-term modulation of calcium (Ca2+) currents (I Ca) was studied in 108CC15 neuroblastoma × glioma hybrid (N×G) cells grown under various culture conditions. The following results were obtained:
-
Article
Abstract of the 68th Meeting (Spring Meeting) 6–9 March 1990, Heidelberg
-
Article
Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture
Three-dimensional cell aggregates (embryoid bodies, EBs) containing clusters of spontaneously beating cardiomyocytes were derived from permanent mouse embryonic stem (ES) cells. Extracellular recordings of th...
-
Article
Outwards currents in embryonic stem cell-derived cardiomyocytes
The aim of the present study was to investigate the expression and functional role of outwards currents during the early stages of cardiomyogenesis. The predominant repolarizing current in early-stage, embryon...
-
Article
Open AccessCav2.3 R-type calcium channels: from its discovery to pathogenic de novo CACNA1E variants: a historical perspective
So-called pharmacoresistant (R-type) voltage-gated Ca2+ channels are structurally only partially characterized. Most of them are encoded by the CACNA1E gene and are expressed as different Cav2.3 splice variants (...
