28 Result(s)
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Article
PCR-Based Ribosomal DNA Detection Technique for Microalga (Heterosigma carterae) Causing Red Tide and Its Application to a Biosensor Using Labeled Probe
A technique for detecting Raphidophycean, a bloom-forming genus of algae, was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the small subunit (SSU) ribo...
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Article
Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...
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Article
Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria
The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...
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Article
Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...
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Article
Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria
The sequential two-step conversion of 4-oxoisophorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) via diyhdrooxoisophorone (DOIP) was achieved using two kinds of thermophiles, Thermomonospora curvat...
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Article
Cloning and expression of Citrobacter freundii hydrogenase genes in Escherichia coli
Escherichia coli mutants deficient in hydrogenase activity (Hyd-) were derived from E. coli C600 by mutagenesis with nitrosoguanidine. Hydrogenase activities of mutant strains; HK-2, HK-7, HK-8, HK-16, HK-23, an...
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Article
Production of l-glutamate by immobilized protoplasts
Protoplasts of Brevibacterium flavum cultured in a medium containing 50 μg·l-1 of biotin were prepared with lysozyme and immobilized in matrices of agar-acetylcellulose filters. The immobilized protoplasts were a...
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Article
Cloning and expression of Citrobacter freundii β-isopropylmalate dehydrogenase gene in both Escherichia coli and Bacillus subtilis
β-Isopropylmalate (IPM) dehydrogenase gene of Citrobacter freundii was cloned in both Escherichia coli and Bacillus subtilis. Plasmid pCBL 1 containing C. freundii β-IPM dehydrogenase gene was isolated using E. c...
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Article
Enzyme sensor for hypoxanthine and inosine determination in edible fish
An enzyme sensor for hypoxanthine (Hx) and inosine (HxR), consisting of an enzyme membrane and an oxygen electrode, was constructed, Xanthine oxidase (XO) and nucleoside phosphorylase (NP) were both immobilize...
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Article
Isolation and partial characterization of large plasmids in hydrogen-evolving bacterium Clostridium butyricum
The hydrogen-evolving bacterium Clostridium butyricum IFO 3847 was found to carry three plasmids: pSSK1, pSSK2, and pSSK3. The molecular weights of pSSK1, 2 and 3, determined by agarose gel electrophoresis and el...
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Article
Sulfite ion sensor with use of immobilized organelle
The sulfite ion sensor consisted of immobilized microsomes and an oxygen electrode. When the sensor was inserted into the sample solution, a steady-state current was obtained within 10 min. A linear relationsh...
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Article
Hybrid urea sensor using nitrifying bacteria
A new urea sensor was developed using immobilized nitrifying bacteria isolated from activated sludges and urease. The sensor was composed of a urease-membrane, a cation exchange membrane, and an alkaline bed (...
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Article
Amperometric determination of sodium nitrite by a microbial sensor
A microbial sensor was prepared to determine sodium nitrite. This microbial sensor consisted of immobilized Nitrobacter sp. and an oxygen electrode. When a sample solution containing sodium nitrite was tested, ni...
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Article
Determination of cell numbers of lactic acid producing bacteria by lactate sensor
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Article
Hydrogen production from glucose by immobilized growing cells ofClostridium butyricum
Clostridium butyricum was immobilized in a porous carrier (acetylcellulose filter) with agar. Addition of peptone to the reaction mixture increased the hydrogen productivity from glucose. The number of cells in ...
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Article
Ultrasound sensitive enzyme-membrane
Glucose oxidase (GOD) was immobilized on an acetylcellulose membrane filter (pore size: 0.45 μm) with dipalmitoyl phosphatidyl-choline (DPPC). The activity of the GOD-DPPC membrane appeared during ultrasonic i...
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Article
Stress sensitive glucose oxidase-nylon membrane
Glucose oxidase (GOD) was immobilized on a nylon membrane. No activity of the GOD-nylon membrane was observed under normal conditions but it appeared when the membrane was mechanically stretched. A linear rela...
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Article
Microbial sensor system which usesMethylomonas sp. for the determination of methane
A microbial sensor system was prepared for the detemination of methane. This microbial sensor consisted of immobilizedMethylomonas sp. and an oxygen electrode. When sample gas containing methane was applied to th...
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Article
Amperometric determination of viable cell numbers based on sensing microbial respiration
Viable cell numbers were determined with an electrode system composed of a membrane filter for retaining microorganisms and an oxygen electrode. The principle of the cell number determination is based on sensi...
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Article
Production of 1,2-epoxyalkanes from 1-alkenes by Nocardia corallina B-276
A gaseous hydrocarbon-assimilating microorganism, Nocardia corallina, grew on 1-alkenes (C3, C4 and C13 to C18) and produced corresponding 1,2-epoxyalkanes. One of the products, 1,2-epoxytetradecane, had a positi...
