16 Result(s)
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Article
Frank Stahl
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Article
Open AccessLow impact of regular PCR testing on presence at work site during the COVID-19 pandemic: experiences during an open observational study in Lower Saxony 2020-21
Since social distancing during the COVID-19-pandemic had a profound impact on professional life, this study investigated the effect of PCR testing on on-site work.
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Open AccessLung Surfactant Accelerates Skin Wound Healing: A Translational Study with a Randomized Clinical Phase I Study
Lung surfactants are used for reducing alveolar surface tension in preterm infants to ease breathing. Phospholipid films with surfactant proteins regulate the activity of alveolar macrophages and reduce inflam...
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Open AccessMicroarray-based screening system identifies temperature-controlled activity of Connexin 26 that is distorted by mutations
Here, we show that human Connexin 26 (hCx26 or Cx26WT) hemichannel opening rapidly enables the transport of small molecules when triggered by temperature and by compensation of the Ca2+ blockade with EDTA. Point ...
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Entwicklung komplexer Screening verfahren am Beispiel von Hsp90
The protein target-microarray assay is a hitherto underestimated method in the investigation of protein-ligand/inhibitor and protein-protein/inhibitor-interactions. It is a versatile and adaptable method that ...
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In vitro toxicological nanoparticle studies under flow exposure
The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitr...
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Open AccessRegulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are large...
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Open AccessDifferent gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: results from a randomized controlled trial
Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in hu...
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Open AccessTranscriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effec...
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Anwendungen von Aptameren in der Mikroarray-Technologie
Die Anwendung von Aptameren in der Mikroarray-Technik ist eine neue, vielversprechende Methode zur Detektion von Proteinen. Aptamer-Mikroarrays können zudem als Screening-Tools für die Untersuchung der Aptamer...
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Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord
Up to 2.8 × 107 fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical co...
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Open AccessLaser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers
Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context, in situ conjugation of nanoparticles with biomolecules via laser ablation in an aque...
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Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression
Characterizing and understanding the functional heterogeneity in a given population on the cellular and molecular level is a great challenge in microbiology. Each microorganism contributes differently to the o...
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Characterization of acetylcholine- and endothelin-induced calcium entry in cultured human ciliary muscle cells
We characterized the effects of acetylcholine and endothelin on cultured human ciliary muscle cells, using the calcium-sensitive dye fura-2 to measure intracellular calcium and intracellular microelectrodes to...
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Na+-dependent HCO 3 − transport and Na+/H+ exchange regulate pH i in human ciliary muscle cells
We investigated intracellular pH (pH i ) regulation in cultured human ciliary muscle cells by means of the pH-sensitive absorbance of 5(and 6)-carboxy-4′,5′-dimethylfluorescein (...
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Regulation of cytoplasmic pH of cultured bovine corneal endothelial cells in the absence and presence of bicarbonate
Intracellular pH (pH i ) in confluent monolayers of cultured bovine corneal endothelial cells was determined using the pH-dependent absorbance of intracellularly trapped 5(and 6)...
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Evidence for Na/H exchange and Cl/HCO3 exchange in A10 vascular smooth muscle cells
In the present study we used the pH sensitive absorbance of 5(and6)-carboxy-4′,5′-dimethylfluorescein to investigate intracellular pH (pHi) regulation in A10 vascular smooth muscle cells: (1) The steady state pHi
