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Article
Apparent incompatibility of plasmid pSfrYC4b of Sinorhizobium fredii with two different plasmids in another strain
Sinorhizobium fredii YC4B is a spontaneous mutant derivative of strain YC4 that is unable to nodulate soybeans. The second-largest plasmid of strain YC4B, termed pSfrYC4b (810 kb), was transferred to S. fredii HN...
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Article
Identification of two novel nodule-specific genes from Astragalus sinicus L. by suppressive subtractive hybridization
To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is ge...
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Article
Effects of the purL Gene Expression Level on the Competitive Nodulation Ability of Sinorhizobium fredii
Purine pathway in Rhizobium is important during the nodulation processes. The purL gene in Sinorhizobium fredii (S. fredii) has been identified to be required for the whole establishment of a nitrogen-fixing nodu...
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Article
A purple acid phosphatase plays a role in nodule formation and nitrogen fixation in Astragalus sinicus
The AsPPD1 gene from Astragalus sinicus encodes a purple acid phosphatase. To address the functions of AsPPD1 in legume-rhizobium symbiosis, its expression patterns, enzyme activity, subcellular localization, and...
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Article
Identification and Symbiotic Phenotype Characterization of an OPDA Reductase Gene AsOPR1 in Chinese Milk Vetch
In higher plants, the 12-oxo-phytodienoic acid reductase (OPR) family mainly consists of two subgroups. OPR3-like enzymes belong to subgroup II, and they were proved to be key functional enzymes participating ...
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Article
A legume kinesin controls vacuole morphogenesis for rhizobia endosymbiosis
Symbioses between legumes and rhizobia require establishment of the plant-derived symbiosome membrane, which surrounds the rhizobia and accommodates the symbionts by providing an interface for nutrient and sig...
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Article
Open AccessScreening of reference genes for microRNA analysis in the study of solider caste differentiation of Formosan subterranean termite Coptotermes formosanus Shiraki
The soldier caste differentiation is a complex process that is governed by the transcriptional regulation and post-transcriptional regulation. microRNAs (miRNAs) are noncoding RNAs that control a wide range of...