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Article
DNA methylation: Bisulphite modification and analysis
DNA methylation is an important epigenetic modification of DNA in mammalian genomes. DNA methylation patterns are established early in development, modulated during tissue-specific differentiation and disrupte...
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Article
Epigenetic remodeling in colorectal cancer results in coordinate gene suppression across an entire chromosome band
We report a new mechanism in carcinogenesis involving coordinate long-range epigenetic gene silencing. Epigenetic silencing in cancer has always been envisaged as a local event silencing discrete genes. Howeve...
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Article
Hypermethylation of the prostacyclin synthase (PTGIS) promoter is a frequent event in colorectal cancer and associated with aneuploidy
Inactivation of specific tumor suppressor genes by transcriptional silencing associated with hypermethylation of the promoter is a common event in cancer. We have applied the amplification of intermethylated s...
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Article
Alterations in the p16INK4a and p53 tumor suppressor genes of hTERT-immortalized human fibroblasts
Exogenous expression of the catalytic subunit of telomerase, hTERT, in a normal human foreskin fibroblast cell strain resulted in telomerase activity and an extended proliferative lifespan prior to a period of...
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Protocol
Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples
It is now well established that, in addition to genetic changes that may include germ line and somatic DNA alterations, cancers can also arise as a result of a series of epigenetic DNA mutations (1). In mammals, ...
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Article
DNA methylation and gene silencing in cancer: which is the guilty party?
The DNA methylation pattern of a cell is exquisitely controlled during early development resulting in distinct methylation patterns. The tight control of DNA methylation is released in the cancer cell characte...
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Article
Hypermethylation trigger of the glutathione-S-transferase gene (GSTP1) in prostate cancer cells
Understanding what triggers hypermethylation of tumour suppressor genes in cancer cells is critical if we are to discern the role of methylation in the oncogenic process. CpG sites in CpG island promoters, tha...
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Article
Detailed methylation analysis of the glutathione S-transferase π (GSTP1) gene in prostate cancer
Glutathione-S-Transferases (GSTs) comprise a family of isoenzymes that provide protection to mammalian cells against electrophilic metabolites of carcinogens and reactive oxygen species. Previous studies have ...
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Article
CpNpG methylation in mammalian cells
In vertebrate DMA, 3% to 5% of cytosine residues are present as 5–methylcytosine, and it is generally accepted that essentially all of this methylation occurs at cytosines which are contained in the symmetrica...
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Article
Hormone Ontogeny in the Ovine Fetus: XIX: The Effect of a Potent Luteinizing Hormone-Releasing Factor Agonist on Gonadotropin and Testosterone Release in the Fetus and Neonate'
ABSTRACT: To investigate further the role of the hypothalamic luteinizing hormone releasing factor (LRF) pulse generator and the pituitary LRF receptor in the regulation of gonadotropin secretion and gonadal s...
