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Article
Isolation of a morphologically and functionally distinct smooth muscle cell type from the intimal aspect of the normal rat aorta. Evidence for smooth muscle cell heterogeneity
Recent studies indicate that the neointima of injured rat arteries is composed of a subpopulation of smooth muscle cells (SMCs) distinct from medial smooth muscle cells. However, SMC diversity in normal adult ...
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Article
Isolation and characterization of vasoformative endothelial cells from the rat aorta
We describe here a modified nonenzymatic method for the isolation of rat aortic endothelial cells with vasoformative properties. Aortic rings placed on plastic or gelatin-coated surfaces generated outgrowths p...
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Article
Ring formation in cultures of rat aortic smooth muscle cells
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Article
Regulation of angiogenesis in vitro by collagen metabolism
The role of collagen in microvascular growth was investigated using the aortic ring model of angiogenesis. Collagen production by vasoformative outgrowths in plasma clot culture of rat aorta was either stimula...
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Article
Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three-dimensional cultures of rat aorta: A comparative study of angiogenesis in Matrigel, collagen, fibrin, and plasma clot
Rings of rat aorta cultured in Matrigel, a reconstituted gel composed of basement membrane molecules, gave rise to three-dimensional networks composed of solid cellular cords and occasional microvessels with s...
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Article
Angiogenesis and the formation of lymphaticlike channels in cultures of thoracic duct
Segments of rat thoracic duct cultured in plasma clot or in collagen gel produced microvascular and fibroblastic outgrowths. Lymphaticlike channels (LLC) with a highly attenuated endothelium, which was barely ...
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Chapter
The Extracellular Matrix Produced during Angiogenesis in Culture
Rings of rat aorta embedded in thick plasma clot and cultured either in Eagle’s minimum essential medium or in Waymouth’s MB 752/1, each supplemented with 20% fetal bovine serum, 50 μg/ml ascorbic acid, and an...
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Article
Interactions between newly formed endothelial channels and carcinoma cells in plasma clot culture
Bovine capillary endothelial cells (BCEC), cultured in suspension on a rotary shaker, formed aggregates ranging from 50 to 300 µm in diameter. In plasma clot these aggregates sprouted in multiple directions an...
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Article
Histotypic angiogenesis in vitro: Light microscopic, ultrastructural, and radioautographic studies
A model for the study of angiogenesis in vitro is described. Rat aortas, cultured in a tridimensional matrix of clotted chick plasma, gave rise to luxuriant outgrowth of vascular channels. We studied this proc...
