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Article
A buckling-based metrology for measuring the elastic moduli of polymeric thin films
As technology continues towards smaller, thinner and lighter devices, more stringent demands are placed on thin polymer films as diffusion barriers, dielectric coatings, electronic packaging and so on. Therefo...
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Article
Analysis of a marsupial MHC region containing two recently duplicated class I loci
A 37-kb cosmid containing two complete major histocompatibility complex (MHC) class I α chain loci from the opossum Monodelphis domestica was isolated, fully sequenced, and characterized. This sequence represents...
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Article
The complexity of expressed kappa light chains in egg-laying mammals
Complementary DNAs encoding immunoglobulin light chains were isolated from two monotreme species, Ornithorhynchus anatinus (duckbill platypus) and Tachyglossus aculeatus (echidna). The sequences of both the varia...
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Article
Isolation of major histocompatibility complex Class I genes from the tammar wallaby (Macropus eugenii)
The major histocompatibility complex (MHC) plays an essential role in the adaptive immune system of vertebrates through antigen recognition. Although MHC genes are found in all vertebrates, the MHC region is d...
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Article
Modo-UG, a marsupial nonclassical MHC class I locus
Modo-UG is a class I gene located in the MHC of the marsupial Monodelphis domestica, the gray, short-tailed opossum. Modo-UG is expressed as three alternatively spliced mRNA forms, all of...
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Article
Open AccessAnalysis of a set of Australian northern brown bandicoot expressed sequence tags with comparison to the genome sequence of the South American grey short tailed opossum
Expressed sequence tags (ESTs) have been used for rapid gene discovery in a variety of organisms and provide a valuable resource for whole genome annotation. Although the genome of one marsupial, the opossum Mono...
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Article
Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences
We report a high-quality draft of the genome sequence of the grey, short-tailed opossum (Monodelphis domestica). As the first metatherian (‘marsupial’) species to be sequenced, the opossum provides a unique persp...
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Article
Open AccessComparative genomic analysis and evolution of the T cell receptor loci in the opossum Monodelphis domestica
All jawed-vertebrates have four T cell receptor (TCR) chains: alpha (TRA), beta (TRB), gamma (TRG) and delta (TRD). Marsupials appear unique by having an additional TCR: mu (TRM). The evolutionary origin of TR...
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Article
Superior mechanical properties of dense and porous organic/inorganic hybrid thin films
The intrinsic mechanical properties of a given material strongly depend upon its chemical nature: the organics tend to be soft, but tough, while the inorganic materials are hard but brittle and are prone to fr...
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Article
On the genomics of immunoglobulins in the gray, short-tailed opossum Monodelphis domestica
Annotated maps of the IGH, IGK, and IGL loci in the gray, short-tailed opossum Monodelphis domestica were generated from analyses of the available whole genome sequence for this species. Analyses of their content...
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Chapter
Marsupial Immunoglobulin and T Cell Receptor Genomics
The development of marsupial genomic resources has provided a wealth of information for the study of the development and function of the adaptive immune system in these remarkable mammals. Here is reviewed the...
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Article
Silsesquioxane-based Photo-Patternable Porous Low-k Dielectric Materials
The future resolution requirements for the semiconductor industry demand advanced lithographic techniques, such as immersion and extreme ultraviolet (EUV) technologies, which will increase the cost of microele...
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Article
Pyrene Fluorescence as a Molecular Probe of Miscibility in Organic/Inorganic Hybrid Nanocomposites Suitable for Microelectronic Applications
Fluorescence spectroscopy has been used to study the miscibility of methyl silsesquioxane (MSSQ)/poly(methyl methacrylate-co-dimethylaminoethyl methacrylate) [P(MMA-co-DMAEMA)] hybrid nanocomposites, which are...
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Article
Open AccessTranscriptomic analysis supports similar functional roles for the two thymuses of the tammar wallaby
The thymus plays a critical role in the development and maturation of T-cells. Humans have a single thoracic thymus and presence of a second thymus is considered an anomaly. However, many vertebrates have mult...
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Article
Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2 and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco
Nornicotine accumulation in tobacco is of concern because nornicotine is a precursor of N-nitrosonornicotine (NNN), a tobacco constituent recognized as a carcinogen by the health community. Nornicotine is derived...
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Article
A VpreB3 homologue in a marsupial, the gray short-tailed opossum, Monodelphis domestica
A VpreB surrogate light (SL) chain was identified for the first time in a marsupial, the opossum Monodelphis domestica. Comparing the opossum VpreB to homologues from eutherian (placental mammals) and avian speci...
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Article
Comparative analysis of the chicken TCRα/δ locus
The access to whole genome sequences has provided the opportunity to study the evolution and organization of immunologically related genes on a large scale. The genes encoding the T cell receptor (TCR) α and δ...
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Article
Recombination, transcription, and diversity of a partially germline-joined VH in a mammal
Full or partially germline-joined V genes have been described in a number of different vertebrate lineages where they can contribute to the expressed antibody repertoire through different mechanisms. Here we d...
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Chapter
The Evolution and Structure of Atypical T Cell Receptors
The T cell receptor structure and genetic organization have been thought to have been stable in vertebrate evolution relative to the immunoglobulins. For the most part, this has been true and the content and o...
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Article
The opossum MHC genomic region revisited
The gray short-tailed opossum Monodelphis domestica is one of the few marsupial species for which a high quality whole genome sequence is available and the major histocompatibility complex (MHC) region has been a...
