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116 Result(s)
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Article
Semi-rational design of L-amino acid deaminase for production of pyruvate and d-alanine by Escherichia coli whole-cell biocatalyst
In our previous study, one-step pyruvate and d-alanine production from d,l-alanine by a whole-cell biocatalyst Escherichia coli expressing l-amino acid deaminase (Pm1) derived from Proteus mirabilis was investiga...
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Article
Metabolic engineering of Escherichia coli for the production of Lacto-N-neotetraose (LNnT)
Lacto-N-neotetraose (LNnT), one of the most important human milk oligosaccharides, can be used as infants’ food additives. Nowadays, extraction, chemical and biological synthesis were utilized to obtain LNnT, whi...
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Article
Metabolic engineering for the synthesis of steviol glycosides: current status and future prospects
With the pursuit of natural non-calorie sweeteners, steviol glycosides (SGs) have become one of the most popular natural sweeteners in the market. The SGs in Stevia are a mixture of SGs synthesized from steviol (...
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Article
Reconstruction of the glutamate decarboxylase system in Lactococcus lactis for biosynthesis of food-grade γ-aminobutyric acid
Gamma-aminobutyric acid (GABA), an important bioactive compound, is synthesized through the decarboxylation of L-glutamate (L-Glu) by glutamate decarboxylase (GAD). The use of lactic acid bacteria (LAB) as cataly...
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Article
Recent advances and challenges in microbial production of human milk oligosaccharides
Human milk oligosaccharides (HMOs) are one of the major differences between livestock milk and human milk, and the prebiotic functions of HMOs have been verified through in vitro and clinical trials. The most ...
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Article
Current advances in design and engineering strategies of industrial enzymes
Enzymes are considered as functional proteins, also known as biocatalysts, which are required for normal cellular function. The commercial production of enzymes with distinct features always remains the top pr...
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Article
Current progress and prospects of enzyme technologies in future foods
Enzyme technologies are widely used in the food industry due to their advantages of high efficiency, specificity, and safety. Recently, “future foods” is emerging as a new research hotspot with healthier foods...
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Article
Combinatorial strategy towards the efficient expression of lipoxygenase in Escherichia coli at elevated temperatures
Lipoxygenases (LOXs) are a family of non-heme iron oxidoreductases, which catalyze the addition of oxygen into polyunsaturated fatty acids. They have applications in the food and medical industries. In most st...
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Article
Towards next-generation model microorganism chassis for biomanufacturing
Synthetic biology provides powerful tools and novel strategies for designing and modifying microorganisms to function as cell factories for biomanufacturing, which is a promising approach for realizing chemica...
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Article
Current advance in biological production of short-chain organic acid
As natural metabolites, organic acids have been widely applied in food, pharmaceutical, and bio-based materials industries. Particularly, the short-chain organic acids, including C2, C3, C4, C5, and C6 organic...
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Article
Metabolic engineering for the production of fat-soluble vitamins: advances and perspectives
Fat-soluble vitamins are vitamins that are insoluble in water, soluble in fat, and organic solvents; they are found in minute amount in various foods. Fat-soluble vitamins, including vitamins A, D, E, and K, h...
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Article
Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of l-DOPA
l-DOPA is a key pharmaceutical agent for treating Parkinson’s, and market demand has exploded due to the aging population. There are several challenges associated with the chemical synthesis of l-DOPA, including ...
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Article
Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches
During fermentation, acid stress caused by the accumulation of acidic metabolites seriously affects the metabolic activity and production capacity of microbial cells. To elucidate the acid stress-tolerance mec...
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Article
Stress tolerance phenotype of industrial yeast: industrial cases, cellular changes, and improvement strategies
Yeast is widely used in the baking, biocontrol, brewing, and bio manufacturing industries. In the baking industry alone, around two million tons of yeast are consumed worldwide every year. While yeast brings d...
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Article
Open AccessCharacterization of a Lactobacillus brevis strain with potential oral probiotic properties
The microflora composition of the oral cavity affects oral health. Some strains of commensal bacteria confer probiotic benefits to the host. Lactobacillus is one of the main probiotic genera that has been used to...
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Article
Improved acid-stress tolerance of Lactococcus lactis NZ9000 and Escherichia coli BL21 by overexpression of the anti-acid component recT
Acid accumulation caused by carbon metabolism severely affects the fermentation performance of microbial cells. Here, different sources of the recT gene involved in homologous recombination were functionally over...
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Article
Transcriptional regulator XYR1 activates the expression of cellobiose synthase to promote the production of cellulase from glucose
To investigate the transcriptional regulation of cellobiose synthase (CBS) in Rhizopus stolonifer.
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Article
Evolutionary engineering of industrial microorganisms-strategies and applications
Microbial cells have been widely used in the industry to obtain various biochemical products, and evolutionary engineering is a common method in biological research to improve their traits, such as high enviro...
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Article
Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast
Yeast has been increasingly used as a host for the expression of enzymes. Compared to other expression systems, the yeast expression system has many advantages including its suitability for large-scale ferment...
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Protocol
Combinatorial Evolution of DNA with RECODE
In past decades, DNA engineering protocols have led to the rapid development of synthetic biology. To engineer the natural proteins, many directed evolution methods based on molecular biology have been present...