40 Result(s)
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Article
Cerebral blood flow changes in hemodialysis and peritoneal dialysis patients: an arterial-spin labeling MR imaging
We used arterial-spin labeling (ASL) MR imaging, a non-invasive technique to evaluate cerebral blood flow (CBF) changes in patients with end-stage renal disease (ESRD) undergoing peritoneal dialysis (PD) and h...
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Article
Expression of Shewanella frigidimarina fatty acid metabolic genes in E. coli by CRISPR/cas9-coupled lambda Red recombineering
To construct a clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system and use this system to obtain a recombinant Escherichia coli strain possessing the fatty acid metabolism genes from ...
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Article
The brain following transjugular intrahepatic portosystemic shunt: the perspective from neuroimaging
Hepatic encephalopathy (HE) is a common complication after implantation of a transjugular intrahepatic portosystemic shunt (TIPS). Neuroimaging offers a variety of techniques for non-invasive evaluation of alt...
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Article
Altered brain functional connectivity in hemodialysis patients with end-stage renal disease: a resting-state functionalMR imaging study
The changes of whole brain functional connectivity in hemodialysis (HD) patients with end-stage renal disease (ESRD) are still unclear, which may be associated with multiple factors, such as elevated neurotoxi...
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Article
Imaging the Cytosolic Drug Delivery Mechanism of HDL-Like Nanoparticles
Molecular therapeutics often require an effective nanoparticle-based delivery strategy to transport them to cytosolic organelles to be functional. Recently, a cytosolic delivery strategy based on the scavenger...
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Article
Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation
Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and ...
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Article
Cloning, expression and stress-respondent transcription of long-chain acyl-coenzyme A synthetase cDNA gene of Nannochloropsis gaditana and its involvement in the biosynthesis of eicosapentaenoic and decosahexaenoic acids
A novel cDNA gene, NgLACS, that encodes a long-chain acyl-CoA sythetase (LACS), was cloned from Nannochloropsis gaditana and characterized. The cDNA was 2,360 bp in length, consisting of an ORF of 1,950 bp, a 5′-...
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Article
Predominant gray matter volume loss in patients with end-stage renal disease: a voxel-based morphometry study
To investigate the pattern of brain volume changes in patients with end-stage renal disease (ESRD) using voxel-based morphometry (VBM) and correlation with clinical and neuropsychological (NP) tests. Fifty sev...
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Article
Transjugular intrahepatic portosystemic shunt induced short- and long-term cerebral blood flow variations in cirrhotic patients: an arterial spin labeling MRI study
Short- and long-term effects of transjugular intrahepatic portosystemic shunt (TIPS) on cerebral blood flow (CBF) in patients with cirrhosis are still unclear. The purpose of this longitudinal study was to exp...
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Article
Peptide-based molecular beacons for cancer imaging and therapy
Peptide-based molecular beacons are Förster resonance energy transfer-based target-activatable probes. They offer control of fluorescence emission in response to specific cancer targets and thus are useful too...
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Article
Cytosolic delivery of LDL nanoparticle cargo using photochemical internalization
Following cellular delivery, most drugs must escape endosomes and lysosomes and reach the cytosol to be effective. This is particularly significant for nanoparticles, which can carry a large drug payload, but ...
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Chapter
Pulsed Gradient Spin-Echo NMR
Translational diffusion (also referred to as self-diffusion, Brownian motion, or random walks) plays a vital role in all sorts of molecular dynamics in biological systems (e.g., ligand–DNA interactions, lipid–...
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Article
Open AccessInvestigating the specific uptake of EGF-conjugated nanoparticles in lung cancer cells using fluorescence imaging
Targeted nanoparticles have the potential to deliver a large drug payload specifically to cancer cells. Targeting requires that a ligand on the nanoparticle surface interact with a specific membrane receptor o...
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Article
Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments
Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To ov...
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Article
Chaotic solutions in the quadratic integrate-and-fire neuron with adaptation
The quadratic integrate-and-fire (QIF) model with adaptation is commonly used as an elementary neuronal model that reproduces the main characteristics of real neurons. In this paper, we introduce a QIF neuron ...
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Article
A tumor mRNA-triggered photodynamic molecular beacon based on oligonucleotide hairpin control of singlet oxygen production
We report a new class of photodynamic molecular beacon (PMB) with tumor specific mRNA-triggered control of singlet oxygen (1O2) production. The beacon contains a single-stranded oligonucleotide linker that forms ...
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Article
Notch activation promotes cell proliferation and the formation of neural stem cell-like colonies in human glioma cells
Since Notch signaling plays a critical role in stem cells and oncogenesis, we hypothesized that Notch signaling might play roles in cancer stem cells and cancer cells with a stem cell phenotype. In this study,...
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Article
Using the singlet oxygen scavenging property of carotenoid in photodynamic molecular beacons to minimize photodamage to non-targeted cells
We recently introduced the concept of photodynamic molecular beacons (PMB) for selective control of photodynamic therapy (PDT). The PMB consists of a peptide linker that is sequence specific to a cancer-associ...
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Article
Stability of Nmi protein is controlled by its association with Tip60
Tip60 exerts diverse biological functions through mechanisms that are either dependent or independent on its intrinsic histone acetyltransferase activity. In the present study, we identified Nmi (N-Myc and STA...
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Article
Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase
Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp...
