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  1. No Access

    Article

    Expression of Shewanella frigidimarina fatty acid metabolic genes in E. coli by CRISPR/cas9-coupled lambda Red recombineering

    To construct a clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system and use this system to obtain a recombinant Escherichia coli strain possessing the fatty acid metabolism genes from ...

    Jun **a, Ling Wang, Jian-bo Zhu, Cheng-jun Sun, Ming-gang Zheng… in Biotechnology Letters (2016)

  2. No Access

    Article

    Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation

    Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and ...

    Yi-jiang Huang, Ling Wang, Ming-gang Zheng, Li Zheng, Yan-li Tong… in Biotechnology Letters (2014)

  3. No Access

    Article

    Cloning, expression and stress-respondent transcription of long-chain acyl-coenzyme A synthetase cDNA gene of Nannochloropsis gaditana and its involvement in the biosynthesis of eicosapentaenoic and decosahexaenoic acids

    A novel cDNA gene, NgLACS, that encodes a long-chain acyl-CoA sythetase (LACS), was cloned from Nannochloropsis gaditana and characterized. The cDNA was 2,360 bp in length, consisting of an ORF of 1,950 bp, a 5′-...

    Ming-gang Zheng, Yi-jiang Huang, Li Zheng, Zhong-tao Sun… in Biotechnology Letters (2014)