34 Result(s)
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Article
David Schrama
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Open AccessRelative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations
Quantification of T-cell receptor (TCR) chain families can be utilized for detection of clonal T-cell populations. Besides southern blotting and antibody-based approaches, quantitative real time PCR (qRT PCR) ...
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Impact of the CCR5 gene polymorphism on the survival of metastatic melanoma patients receiving immunotherapy
Chemokines influence both tumor progression and anti-tumor immune response. A 32-bp-deletion polymorphism in the chemokine receptor 5 gene (CCR5Δ32) has been shown to result in a non-functional protein. This s...
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Immunological tumor destruction in a murine melanoma model by targeted LTα independent of secondary lymphoid tissue
We previously demonstrated that targeting lymphotoxin α (LTα) to the tumor evokes its immunological destruction in a syngeneic B16 melanoma model. Since treatment was associated with the induction of peritumor...
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Anti-cancer therapies targeting the tumor stroma
For anti-tumor therapy different strategies have been employed, e.g., radiotherapy, chemotherapy, or immunotherapy. Notably, these approaches do not only address the tumor cells themselves, but also the tumor ...
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Dendritic cell based antitumor vaccination: impact of functional indoleamine 2,3-dioxygenase expression
Recent reports have demonstrated that the enzyme indoleamine 2,3-dioxygenase (IDO) is upregulated in human dendritic cells (DCs) upon in vitro maturation. IDO is supposed to convey immunosuppressive effects by...
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Therapeutic efficacy of tumor-targeted IL2 in LTα−/− mice depends on conditioned T cells
An effective immunological eradication of tumors by the adaptive immune system depends on T cell priming, expansion of specific T cells and their effector function. It has been shown that either step may be im...
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Identification of identical TCRs in primary melanoma lesions and tumor free corresponding sentinel lymph nodes
It is generally believed that priming of efficient T-cell responses takes place in peripheral lymphoid tissues. Although this notion has been rigidly proven for infectious diseases, direct evidence for lymph n...
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Tumor stroma-associated antigens for anti-cancer immunotherapy
Immunotherapy has been widely investigated for its potential use in cancer therapy and it becomes more and more apparent that the selection of target antigens is essential for its efficacy. Indeed, limited cli...
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Expression pattern of the lymphatic and vascular markers VEGFR-3 and CD31 does not predict regional lymph node metastasis in cutaneous melanoma
Malignant melanoma of the skin preferentially metastasises via the lymphatic system. Novel molecular biomarkers, which are involved in malignant transformation, proliferation, angiogenesis and lymphangiogenesi...
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Antibody targeted drugs as cancer therapeutics
One way to maximize the effect and minimize the side effects of cancer therapeutics is the targeting of drugs to the tumor by using the specificity of antibodi...
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Cytoplasmic and nuclear expression of survivin in melanocytic skin lesions
Survivin, a member of the inhibitors of apoptosis protein family, regulates both cellular proliferation and apoptotic cell death. While the human survivin gene is highly expressed in the develo** fetus, in a...
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Open AccessT-cell clonotypes in cancer
Cells of the immune system spontaneously recognize autologous tumor cells and T cells are believed to be the main effector cells for the immune surveillance of cancer. Recent advances in our understanding of b...
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Distribution of telomeric (TTAGGG)n sequences in avian chromosomes
The physical ends of mammalian and other vertebrate chromosomes consist of tandemly repeated (TTAGGG)n hexamers, nucleating a specialized telomeric structure. However, (TTAGGG)n sequences can also occur at non-t...
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Comparative delineation of T cell clonotypes in coexisting syngeneic B16 melanoma
B16 is a murine melanoma of C57Bl/6 origin, which rapidly develops as a tumor when inoculated into syngeneic immunocompetent hosts. Nevertheless, B16 tumors are considered to be immunogenic since tumor regres...
