Abstract
This study investigates the genotype × year interaction in tobacco plants facing the threat of Broomrape (Orobanche spp.), a parasitic weed impacting tobacco production. Over two years, 67 tobacco genotypes were assessed separately under normal and O. cernua-infected conditions using a randomized complete block design with three replications. Measurements included plant height, leaf area, number of leaves, leaf fresh weight, and leaf dry weight for tobacco plants. Additionally, parameters for O. cernua, such as the number of broomrapes per pot, broomrape fresh weight, and broomrape dry weight, were recorded. Mixed linear analysis of variance revealed significant differences (LRTenv) for all traits, signifying distinctions between normal and infected conditions. LRTge, indicating genotype × year interaction, was significant for plant height and leaf dry weight. Notably, the study identified tobacco genotypes with stable leaf dry weight over two years under both conditions, with G28 and G29 displaying the highest values. Broomrape growth characteristics varied among tobacco genotypes, and immune types such as G11, G17, and G59 were identified. GGE biplot analysis highlighted differences in tobacco germplasm performance across years and planting conditions. The stability parameter (weighted average of absolute scores, WAASB) was computed, and a biplot of leaf dry weight × WAASB identified G18, G1, G6, G65, G25, G66, G67, G14, and G11 as top-performing genotypes. Using MTSI as a selection index, G16 emerged as the most ideal stable genotype, followed by G29, G25, G11, G4, G65, G28, G64, G44, and G41, exhibiting favorable selection differentials for all traits. This study successfully identifies genotypes with notable resistance levels and stability across diverse years, offering valuable insights for tobacco breeding programs.
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References
Amri M, Abbes Z, Trabelsi I, Ghanem ME, Mentag R, Kharrat M (2021) Chlorophyll content and fluorescence as physiological parameters for monitoring Orobanche foetida Poir. infection in faba bean. PLoS ONE 16(5):e241527. https://doi.org/10.1371/journal.pone.0241527
Benakanahalli NK, Sridhara S, Ramesh N, Olivoto T, Sreekantappa G, Tamam N, Abdelmohsen SA (2021) A framework for identification of stable genotypes basedon MTSI and MGDII indexes: An example in guar (Cymopsis tetragonoloba L.). Agronomy 11(6):1221. https://doi.org/10.3390/agronomy11061221
Buschmann H, Gonsior G, Sauerborn J (2005) Pathogenicity of branched broomrape (Orobanche ramosa) populations on tobacco cultivars. Plant Pathol 54(5):650–656. https://doi.org/10.1111/j.1365-3059.2005.01211.x
Darvishzadeh R (2016) Genetic variability, structure analysis, and association map** of resistance of resistance to broomrape (Orobanche aegyptiaca Pers.) in tobacco. J Agric Sci Technol 18(5):1419–1429
Darvishzadeh R, Alavi R, Sarrafi A (2010) Resistance to powdery mildew (Erysiphe cichoracearum DC.) in oriental and semi-oriental tobacco germplasm under field conditions. J Crop Improv 24(2):122–130. https://doi.org/10.1080/15427520903559425
Davalieva K, Maleva I, Filiposki K, Spiroski O, Efremov GD (2010) Genetic variability of Macedonian tobacco varieties determined by microsatellite marker analysis. Diversity 2(4):439–449. https://doi.org/10.3390/d2040439
El-Aty A, El-Galaly OA, Soliman AAM (2016) Heterosis and combining ability for yield, yield components and inheritance of tolerance to Orobanche in Faba bean. Egypt J Plant Breed 203(3832):1–16. https://doi.org/10.12816/0031711
En-nahli Y, Hejjaoui K, Mentag R, Es-safi NE, Amri M (2023) Large field screening for resistance to broomrape (Orobanche crenata Forsk.) in a global lentil diversity panel (GLDP)(lens culinaris Medik.). Plants 12(10):2064. https://doi.org/10.3390/plants12102064
Fernandez-Aparicio M, Moral A, Kharrat M, Rubiales D (2012) Resistance against broomrapes (Orobanche and Phelipanche spp.) in faba bean (Vicia faba) based in low induction of broomrape seed germination. Euphytica 186:897–905. https://doi.org/10.1007/s10681-012-0686-0
Fernandez-Aparicio M, Reboud X, Gibot-Leclerc S (2016) Broomrape weeds. Underground mechanisms of parasitism and associated strategies for their control: A review. Front Plant Sci 19(7):135. https://doi.org/10.3389/fpls.2016.00135
Habtegebriel HM (2022) Adaptability and stability for soybean yield by AMMI and GGE models in Ethiopia. Front Plant Sci 13:950992
Hatami Maleki H, Karimzadeh G, Darvishzadeh R, Sarrafi A (2011) Correlation and sequential path analysis of some agronomic traits in tobacco (Nicotiana tabaccum L.) to improve dry leaf yield, Aust J Crop Sci 5(12):1644–1648
Kurt D, Yilmaz G, Kinay A (2020) Effects of environmental variations on yield of oriental tobaccos. Int J Agric Wild Sci 6:310–324
Kurt D, Yilmaz G, Kinay A (2021) GE interaction and stability analysis in some Basma type oriental tobacco (Nicotiana tabacum L.) lines. J Agric Sci Bilim Derg 27(3):312–320. https://doi.org/10.15832/ankutbd.680854
Maalouf F, Khalil S, Ahmed S, Akintunde AN, Kharrat M, El Shama’a K, Malhotra RS (2011) Yield stability of faba bean lines under diverse broomrape prone production environments. Field Crop Res 124(3):288–294. https://doi.org/10.1016/j.fcr.2011.06.005
Mortensen DA, Bastiaans L, Sattin MM (2000) The role of ecology in the development of weed management systems: an outlook. Weed Res 40(1):49–62. https://doi.org/10.1046/j.1365-3180.2000.00174.x
Nourollahi F, Mohammaddust-Chamanabad HR, Hasanpanah D, Anvar M (2019) Evaluating the competitive ability of potato cultivars with weeds. Appl Ecol Environ Res 17(4):8835–8845. https://doi.org/10.15666/aeer/1704_88358845
Oghan HA, Bakhshi B, Rameeh V, Tabrizi HZ, Faraji A, Ghodrati G, Dalili A (2023) Comparative study of univariate and multivariate selection strategies based on an integrated approach applied to oilseed rape breeding. Crop Sci. https://doi.org/10.1002/csc2.21104
Olivoto T, Lúcio ADC (2020) metan: An R package for multi-environment trial analysis. Methods Ecol Evol 11(6):783–789. https://doi.org/10.1111/2041-210x.13384
Olivoto T, Lúcio AD, da Silva JA, Marchioro VS, de Souza VQ, Jost E (2019a) Mean performance and stability in multi-environment trials I: combining features of AMMI and BLUP techniques. Agron J 111(6):2949–2960. https://doi.org/10.2134/agronj2019.03.0220
Olivoto T, Lúcio AD, da Silva JA, Sari BG, Diel MI (2019b) Mean performance and stability in multi-environment trials II: Selection based on multiple traits. Agron J 111(6):2961–2969. https://doi.org/10.2134/agronj2019.03.0221
Pérez-de-Luque A, Moreno MT, Rubiales D (2008) Host plant resistance against broomrapes (Orobanche spp.): defence reactions and mechanisms of resistance. Ann Appl Biol 152(2):131–141. https://doi.org/10.1111/j.1744-7348.2007.00212.x
Piepho HP (1994) Best linear unbiased prediction (BLUP) for regional yield trials: a comparison to additive main effects and multiplicative interaction (AMMI) analysis. Theor Appl Genet 89:647–654. https://doi.org/10.1007/bf00222462
Porkabiri Z, Sabaghnia N, Ranjbar R, Maleki HH (2019) Genetic variation of some tobacco (Nicotiana tabacum L.) genotypes by morphological traits. Sci Agric Bohem 50(1):1–7. https://doi.org/10.2478/sab-2019-0001
Rubiales D, Fernández-Aparicio M, Wegmann K, Joel DM (2009) Revisiting strategies for reducing the seedbank of Orobanche and Phelipanche spp. Weed Res 49:23–33. https://doi.org/10.1111/j.1365-3180.2009.00742.x
Rubiales D, Flores F, Emeran AA, Kharrat M, Amri M, Rojas-Molina MM, Sillero JC (2014) Identification and multi-environment validation of resistance against broomrapes (Orobanche crenata and Orobanche foetida) in faba bean (Vicia faba). Field Crop Res 166:58–65. https://doi.org/10.1016/j.fcr.2014.06.010
Rubiales D, Moral A, Flores F (2022) Agronomic performance of broomrape resistant and susceptible faba bean accession. Agronomy 12(6):1421. https://doi.org/10.3390/agronomy12061421
Saeidnia F, Taherian M, Nazeri SM (2023) Graphical analysis of multi-environmental trials for wheat grain yield based on GGE-biplot analysis under diverse sowing dates. BMC Plant Biol 23(1):1–16. https://doi.org/10.1186/s12870-023-04197-9
Schneeweiss GM, Palomeque T, Colwell AE, Weiss-Schneeweiss H (2004) Chromosome numbers and karyotype evolution in holoparasitic Orobanche (Orobanchaceae) and related genera. Am J Bot 91(3):439–448. https://doi.org/10.3732/ajb.91.3.439
Sellami MH, Pulvento C, Lavini A (2021) Selection of suitable genotypes of lentil (Lens culinaris Medik.) under rainfed conditions in south Italy using multi-trait stability index (MTSI). Agronomy 11(9):1807. https://doi.org/10.3390/agronomy11091807
Shi B, Zhao J (2020) Recent progress on sunflower broomrape research in China. OCL 27:30. https://doi.org/10.1051/ocl/2020023
Sillero JC, Villegas-Fernández AM, Thomas J, Rojas-Molina MM, Emeran AA, Fernández-Aparicio M, Rubiales D (2010) Faba bean breeding for disease resistance. Field Crop Res 115(3):297–307. https://doi.org/10.1016/j.fcr.2009.09.012
Singamsetti A, Zaidi PH, Seetharam K, Vinayan MT, Olivoto T, Mahato A, Shikha K (2023) Genetic gains in tropical maize hybrids across moisture regimes with multi-trait-based index selection. Front Plant Sci 14:1147424. https://doi.org/10.3389/fpls.2023.1147424
Taleghani D, Rajabi A, Saremirad A, Fasahat P (2023) Stability analysis and selection of sugar beet (Beta vulgaris L.) genotypes using AMMI, BLUP, GGE biplot and MTSI. Sci Rep 13(1):10019. https://doi.org/10.21203/rs.3.rs-2769933/v1
Tokasi S, Bannayan Aval M, Mashhadi HR, Ghanbari A (2014) Screening of resistance to Egyptian broomrape infection in tomato varieties. Planta Daninha 32:109–116. https://doi.org/10.1590/s0100-83582014000100012
Trabelsi I, Abbes Z, Amri M, Kharrat M (2016) Study of some resistance mechanisms to Orobanche spp. infestation in faba bean (Vicia faba L.) breeding lines in Tunisia. Plant Prod Sci 19(4):562–573. https://doi.org/10.1080/1343943x.2016.1221734
Valderrama MR, Román B, Satovic Z, Rubiales D, Cubero JI, Torres AM (2004) Locating quantitative trait loci associated with Orobanche crenata resistance in pea. Weed Res 44(4):323–328. https://doi.org/10.1111/j.1365-3180.2004.00406.x
Van Bruggen AH, He MM, Shin K, Mai V, Jeong KC, Finckh MR, Morris JG Jr (2018) Environmental and health effects of the herbicide glyphosate. Sci Total Environ 616:255–268. https://doi.org/10.1016/j.scitotenv.2017.10.309
Whitney PJ, Carsten C (1981) Chemotropic response of broomrape radicles to host root exudates. Ann Bot 48(6):919–921. https://doi.org/10.1093/oxfordjournals.aob.a086201
Yan W, Kang MS (2002) GGE biplot analysis: a graphical tool for breeders, geneticists, and agronomists. CRC https://doi.org/10.1201/9781420040371
Zand E, Beckie HJ (2002) Competitive ability of hybrid and open-pollinated canola (Brassica napus) with wild oat (Avena fatua). Can J Plant Sci 82(2):473–480. https://doi.org/10.4141/p01-149
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H. Hatami Maleki: Conceptualization, Methodology, Formal analysis and investigation, Writing—original draft preparation, Writing—review and editing, Supervision; R. Darvishzadeh: Conceptualization, Methodology, Supervision; H. Zeinalzadeh-Tabrizi: Formal analysis and investigation, Writing—review and editing.
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H. Hatami Maleki, R. Darvishzadeh and H. Zeinalzadeh-Tabrizi declare that they have no competing interests.
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Hatami Maleki, H., Darvishzadeh, R. & Zeinalzadeh-Tabrizi, H. Identification of Resistance Sources Against Orobanche Cernua in Tobacco Germplasm. Journal of Crop Health 76, 701–711 (2024). https://doi.org/10.1007/s10343-024-00987-9
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DOI: https://doi.org/10.1007/s10343-024-00987-9