Abstract
Biofilm-forming Staphylococcus aureus can easily accumulate on various food contact surfaces which induce cross-contamination and are difficult to eliminate in the food industry. This study aimed to evaluate the anti-biofilm effects of natural product biochanin A against S. aureus. Results showed that biochanin A effectively eradicated established S. aureus biofilms on different food-contact materials. Fluorescence microscopic analyses suggested that biochanin A disintegrated the established biofilms by dissociate extracellular polymeric substance (EPS) in matrix. In addition, biochanin A at the sub-MIC concentration also effectively inhibited the biofilm formation by regulating the expression of biofilm-related genes (icaA, srtA, eno) and suppressing the release of EPS in biofilm matrix. Molecular docking also demonstrated that biochanin A conducted strong interactions with biofilm-related proteins (Ica A, Sortase A, and Enolase). These findings demonstrated that biochanin A has the potential to be developed as a potent agent against S. aureus biofilm in food industries.
Key points
• Anti-biofilm effect of biochanin A against S. aureus was revealed for the first time.
• Biofilm of S. aureus on various food-contact surfaces were efficiently eradicated.
• Biochanin A prevented S. aureus biofilm formation via reducing EPS production.
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Data availability
The data that support the findings of this study are available on request from the corresponding author.
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Funding
The work was supported by the National Key Research and Development Program of China (No. 2018YFC1602202), the National Natural Science Foundation of China (No. 31772082), and the 13th Five-Year Plan Science and Technology Project of the Education Department of Jilin Province (JJKH20201017KJ).
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BX, DY, and GN conceived and designed research and wrote the manuscript. MR and SY conducted experiments. ZY and ZT analyzed data. All authors read and approved the manuscript.
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Bai, X., Shen, Y., Zhang, T. et al. Anti-biofilm activity of biochanin A against Staphylococcus aureus. Appl Microbiol Biotechnol 107, 867–879 (2023). https://doi.org/10.1007/s00253-022-12350-x
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DOI: https://doi.org/10.1007/s00253-022-12350-x