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Contribution à l’étude de làabsorption du saccharose par des tissus de tubercule de pomme de terre adulte (Solatium tuberosum L.) modalités et influence des phytohormones

Absorption of sucrose by tissues of adult tubers of potato(Solatium tuberosum L.). modalities and influence of phytohormones

  • Published:
Biologia Plantarum

Abstract

Sugar uptake by potato tuber discs was studied. Discs were used “fresh” or after a 24-h ageing period. It was shown that ageing increases (by 3 to 4 times) the rate of glucose and sucrose uptake. Sucrose uptake by fresh tissues was insensitive to the presence of glucose or fructose while a competitive effect was observed after ageing. This indicates the development of an invertase activity, which was inhibited by tris-buffer. Sucrose and glucose uptake by aged discs was dependent on cellular metabolism as shown by the sensitivity to low temperature and metabolic inhibitors (NaCN, DNP, CCCP). Involvement of thiol groups was demonstrated by the inhibition with NEM and PCMBS. Orthovanadate, which decreases phosphate uptake by 85 % (Poderet al. 1986) did not produce any effect on glucose and sucrose uptake by aged tissues. Fusicoccin produced only a slight stimulation (15 %). These results argue in favour of the involvement of specific ATPases in ion and sugar uptake. No involvement of a redox system was observed. ABA and BAP inhibited the uptake induced by ageing but had no effect on the endogenous sugar content. BAP would act by its effect on the amount of ATP while ABA would act at the membrane level. The results are discussed in relation to the mechanisms of transfer of glucosyl groups and to the transport of sucrose by the symplasmic pathway. Reçule

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Abbreviations

ABA:

acide abscissique

BAP:

benzylaminopurine

CCCP:

cyanure de carbonyl-m-chlorophénylhydrazone

CHM:

cycloheximide

DCCD:

N,N’-dicyclohexyl-carbodiimide

DES:

diéthylstilbestrol

DNP:

2,4-dinitrophénol

FC:

fusicoccine

GA:

acide gibbérellique

IAA:

acide β-indolylacétique

MES:

acide 2(-N-morpholino)éthane-sulphonique

NEM:

N-éthylmaléimide

PCMBS:

acidep-chloromercuribenzénesulphonique

Tris:

tris(hydroxyméthyl)aminométhane

Réferences

  • Ap Rees, T., Morrell, S.: Carbohydrate metabolism in develo** potatoes. - Amer. Potato J.67: 835- 884, 1990.

    CAS  Google Scholar 

  • Bergmeyer, H.U., Bernt, E.: Sucrose. - In: Bergmeyer, H.U.: (ed.): Methods of Enzymatic Analysis. Vol. 3. Pp. 1176–1179. Academic Press, New York - London 1974.

    Google Scholar 

  • Bergmeyer, H.U., Bernt, E., Schmidt, F., Stork, H.: Glucose. - In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Vol. 3. Pp. 1196–1201. Academic Press, New York - London 1974.

    Google Scholar 

  • Dahse, I., Bernstein, M., Müller, E., Petzold, U.: On possible functions of electron transport in the plasmalemma of plant cells. - Biochem. Physiol. Pflanzen185: 145 -180, 1989.

    CAS  Google Scholar 

  • Davies, H.V., Ross, H.A.: Hydrolytic and phosphorolytic enzyme activity and reserve mobilisation in sprouting tubers of potato (Solanum tuberosum L.). - Plant Physiol.186: 387–396, 1987.

    Google Scholar 

  • Delrot, S., Bonnemain, J.L.: Mechanism and control of phloem transport. - Physiol. vég.29: 199–220, 1985.

    Google Scholar 

  • Ewing, E.E.: The role of hormones in potato (Solanum tuberosum L.) tuberization. - In: Davies, P.J. (ed.): Plant Hormones and Their Role in Plant Growth and Development. Pp. 515–538. Martinus Nijhoff Publ., Dordrecht 1987.

    Google Scholar 

  • Felle, H., Bentrup, F.W.: A study of the primary effect of the uncoupler carbonyl cyanidem- chlorophenyl-hydrazone on membrane potential and conductance inRiccia fluitans. - Biochim. biophys. Acta464: 179–187, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Giaquinta, R.T., Lin, W., Sadler, N.L., Franceschi, V.R.: Pathway of phloem unloading of sucrose in corn roots. - Plant Physiol.72: 362–367, 1983.

    PubMed  CAS  Google Scholar 

  • Glasziou, K.T., Gayler, K.R.: Storage of sugars in stalks of sugar cane. - Bot. Rev.38:.471–490, 1972.

    Article  Google Scholar 

  • Hager, A., Berthold, W., Biber, W., Edel, H.G., Lanz, C., Schiebel, G.: Primary and secondary energized ion-translocating systems on membranes of plant cells. - Ber. deut. bot. Ges.99: 281–295, 1986.

    Google Scholar 

  • Hayes, P.M., Patrick, J.W.: Photosynthate transport in stems ofPhaseolus vulgaris L. treated with gibberellic acid, indol-3-acetic acid or kinetin. - Planta166: 371–379, 1985.

    Article  CAS  Google Scholar 

  • Ho, L.C.: Sink strength and sugar metabolism. - Annu. Rev. Plant Physiol.39: 355–378, 1988.

    Article  CAS  Google Scholar 

  • Hourmant, A.: Influence de quelques phytohormones sur l’absorption et sur le métabolisme du phosphore au niveau des tissus de tubercules de pomme de terre. - Thèse Doctorat d’Etat. Brest 1979.

  • Hourmant, A., Penot, M.: Action de quelques phytohormones sur la respiration et l’absorption du phosphate par des disqucs de tubercules de pomme de terre en survie. - Physiol. Plant.44: 278- 282, 1978.

    Article  CAS  Google Scholar 

  • Hourmant, A., Pradet. A., Penot, M.: Action de la benzylaminopurine sur le métabolisme des composes phosphorylées des disques de tubercule de pomme de terre en survie. Effect sur la respiration et la teneur en nucléotides adényliques. - Physiol. vég. 17: 483–499, 1979.

    CAS  Google Scholar 

  • Kahl, G.: Metabolism in plant storage tissue slices. - Bot. Rev.40: 263–314, 1974.

    Article  CAS  Google Scholar 

  • Kato, T., Kubota, S.: Properties of invertases in sugar storage tissues of citrus fruit and changes in their activities during maturation. - Physiol. Plant42: 67–72, 1978.

    Article  CAS  Google Scholar 

  • Komor, E., Thorn, M., Maretzki, A.: The mechanism of sugar uptake by sugar cane suspension cells. -Planta153: 181–192, 1981.

    Article  CAS  Google Scholar 

  • Lin, W.: Isolation of NADH oxidation system from the plasmalemma of corn root protoplastes. - Plant Physiol.70: 326–328, 1982a.

    Article  PubMed  CAS  Google Scholar 

  • Lin, W.: Response of corn root protoplasts to exogenous NADH: oxygen consumption, ion uptake, and membrane potential. - Proc. nat. Acad. Sci. USA79: 3773–3776, 1982b.

    Article  PubMed  CAS  Google Scholar 

  • Lin, W.: Further characterization on the transport property of plasmalemma NADH oxidation system in isolated corn root protoplasts. -Plant Physiol.74: 219–222, 1984.

    PubMed  CAS  Google Scholar 

  • Lorenc-Pluciňska, G., Ziegler, H.: Interaction of membrane effectors and sulfite in sucrose uptake ofRicinus communis cotyledons. - J. Plant Physiol.133: 96–102, 1988.

    Google Scholar 

  • Mares, J.J.: Sowokinos, J.R., Hawker, J.S.: Carbohydrate metabolism in develo** potato tubers. - In: Li, P.H. (ed.): Potato Physiology. Pp. 279–327. Academic Press, London 1985.

    Google Scholar 

  • Marré, E.: Fusicoccin: A tool in plant physiology. - Annu. Rev. Plant Physiol.30: 273–288, 1979.

    Article  Google Scholar 

  • Martinola, E., Kaiser, G., Schramm, M.J., Heber, U.: Sugar transport across the plasmalemma and the tonoplast of barley mesophyll protoplasts. Evidence for different transport systems. - Plant Physiol.131:467–478, 1987.

    CAS  Google Scholar 

  • Minchin, P.E.H., Mc Naughton, G.S.: Phloem loading within the seed coats ofPisum sativum observed using surgically modified seeds. - J. exp. Bot.37: 1151–1163, 1986.

    Article  Google Scholar 

  • Morris, D.A., Arthur, E.D.: Effects of gibberellic acid on patterns of carbohydrate distribution and acid invertase activity inPhaseolus vulgaris. - Physiol. Plant.65: 257–262, 1985.

    Article  CAS  Google Scholar 

  • Oparka, K.J.: Phloem unloading in the potato tuber. Pathways and sites of ATPase. - Protoplasma131:201-210.

  • Penot, M., Beraud, J.: Hormone-directed transport in isolated leaves ofPelargonium zonale - Relation with other processes. - Plant Growth Regulators (Sofia)3: 302–309, 1983.

    Google Scholar 

  • Poder, D., Penot, M.: Propriétés du système de transport du phosphate des tissus de tubercules de pomme te terre en survie: effect du pH et de divers inhibiteurs métaboliques. - Physiol. vég.24: 551–557, 1986.

    CAS  Google Scholar 

  • Poder, D., Penot, M.: The effect of vanadate on phosphate transport in aged potato tissue (Solanum tuberosum). - J. exp. Bot.43: 189–193, 1992.

    Article  CAS  Google Scholar 

  • Poder, D., Suleiman, S., Penot, M.: Phosphate transport in potato cuttings. Effect of GA and ABA. -Physiol. Plant.72: 385–388, 1988.

    Article  CAS  Google Scholar 

  • Porter, G.A., Knievel, D.P., Shannon, J.C.: Sugar efflux from maize pedicel tissue. - Plant Physiol.77:524–531, 1985.

    PubMed  CAS  Google Scholar 

  • Saftner, R.A., Wyse, R.E.: Effect of plant hormones on sucrose uptake by sugar beet root tissue discs. - Plant Physiol74: 951–955, 1984.

    PubMed  CAS  Google Scholar 

  • Serrano, R.: Plasma membrane ATPase. - In: Larsson, C., Møller, I.M. (ed.): The Plant Plasma Membrane. Pp. 127–153. Springer-Verlag, Berlin - Heidelberg 1990.

    Google Scholar 

  • Shaner, D.L., Mertz, S.M., Arntzen, C.J.: Inhibition of ion accumulation in maize roots by abscisic acid. -Planta122: 79–90, 1975.

    Article  CAS  Google Scholar 

  • Sowokinos, J.R., Varns, J.L.: Induction of sucrose synthase in potato tissue culture: effect of carbon source and metabolic regulators on sink strength. - Plant Physiol.139: 672–679, 1992.

    CAS  Google Scholar 

  • Suleiman. S., Hourmant, A., Penot, M.: Influence de l’acide abscissique sur le transport d’ions inorganiques chez la pomme de terre(Solanum tuberosum). Etude comparée avec quelques autres phytohormones. - Biol. Plant.32: 128–137, 1990

    Article  CAS  Google Scholar 

  • Suleiman, S., Hourmant, A., Penot, M.: Influence of abscisic on K+ absorption by leaf discs ofSolanum tuberosum. - Biol. Plant.33: 49–57, 1991.

    Article  CAS  Google Scholar 

  • Sze. H: H+-translocating ATPases: advances using membrane vesicles. -Annu. Rev. Plant Physiol.36: 175–208, 1985.

    Article  CAS  Google Scholar 

  • Van Steveninck, F.M.: The “washing” or “aging” phenomenon in plant tissues. - Annu Rev. Plant Physiol.26: 237 - 258, 1975.

    Article  Google Scholar 

  • Vaughan, D., Mac Donald, J.R.: Development of soluble and insoluble invertase activity in washed storage tissue slices. -Plant Physiol.42: 456–458, 1967.

    PubMed  CAS  Google Scholar 

  • Walker, R.R., Leigh, R.A., Mg-dependent, cation-stimulated inorganic pyrophosphate associated from storage roots of red beets(Beta vulgaris L.). -Planta153: 150–155, 1981.

    Article  CAS  Google Scholar 

  • Wolswinkel, P.: The role of maternal tissues in the sink control of assimilate transport into develo** seeds. - Plant Physiol. Biochem.25: 557–566, 1987.

    CAS  Google Scholar 

  • **a, J.H., Saglio, P.H., Characterization of the hexose transport system in maize root tips. - Plant Physiol.88: 1015–1020, 1988.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboraloire de Physiologie Végétale, Faculté des Sciences, Université de Bretagne Occidentale, 29275, Brest Cédex, France

    M. Penot, A. Hourmant & A. Reddahi

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  1. M. Penot
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  2. A. Hourmant
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  3. A. Reddahi
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Penot, M., Hourmant, A. & Reddahi, A. Contribution à l’étude de làabsorption du saccharose par des tissus de tubercule de pomme de terre adulte (Solatium tuberosum L.) modalités et influence des phytohormones. Biol Plant 35, 365–380 (1993). https://doi.org/10.1007/BF02928512

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