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Mg2+-Dependent, cation-stimulated inorganic pyrophosphatase associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.)

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Abstract

Vacuoles isolated from storage roots of red beet (Beta vulgaris L.) posess a Mg2+-dependent, alkaline pyrophosphatase (PPase) activity which is further stimulated by salts of monovalent cations. The requirement for Mg2+ is specific. Mn2+ and Zn2+ permitted only 20% and 12%, respectively, of the PPase activity obtained in the presence of Mg2+ while Ca2+, Co2+ and Cu2+ were ineffective. Stimulation of Mg2+-PPase activity by salts of certain monovalent cations was due to the cation and the order of effectiveness of the cations tested was K+=Rb+=NH +4 >Cs+. Salts of Li+ and Na+ inhibited Mg2+-PPase activity by 44% and 24%, respectively. KCl-stimulation of Mg2+-PPase activity was maximal with 60–100 mM KCl. There was a sigmoidal relationship between PPase activity and Mg2+ concentrations which resulted in markedly non-linear Lineweaver-Burk plots. At pH 8.0, the optimal [Mg2+]:[PPi] ratio for both Mg2+-PPase and (Mg2++KCl)-PPase activities was approximately 1:1, which probably indicates MgP2O7 2- is the true substrate.

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Abbreviations

BSA:

bovine serum albumen

EDTA:

ethylenediamine tetra-acetic acid, disodium salt

MES:

2-(N-morpholino)ethanesulphonic acid

Mg 2+T :

total magnesium

Pi :

inorganic phosphate

PPase:

inorganic pyrophosphatase

PPi :

inorganic pyrophosphate

TCA:

trichloroacetic acid

Tris:

tris(hydroxymethyl)methylamine

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Author notes

  1. Robert R. Walker (Recipient of C.S.I.R.O. Postdoctoral Studentship)

    Present address: Division of Horticultural Research, C.S.I.R.O., 3505, Merbein, Vic., Australia

  2. Roger A. Leigh (Royal Society Pickering Research Fellow)

    Present address: Soils and Plant Nutrition Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK

Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    Robert R. Walker (Recipient of C.S.I.R.O. Postdoctoral Studentship) & Roger A. Leigh (Royal Society Pickering Research Fellow)

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  1. Robert R. Walker
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Walker, R.R., Leigh, R.A. Mg2+-Dependent, cation-stimulated inorganic pyrophosphatase associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.). Planta 153, 150–155 (1981). https://doi.org/10.1007/BF00384096

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