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Development and validation of cost-effective SYBR Green-based RT-qPCR and its evaluation in a sample pooling strategy for detecting SARS-CoV-2 infection in the Indonesian setting
A low-cost SYBR Green-based RT-qPCR method to detect SARS-CoV-2 were developed and validated. Primers targeting a conserved and vital region of the N...
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Quantitative PCR of Alu Repeats Using PowerUp™ SYBR® Green Master Mix
Quantitative PCR is one of the fundamental steps performed when processing routine casework in a forensic laboratory. Quantitative PCR of Alu repeats...
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Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats
Feline parvovirus causes infectious diseases, and Chaphamaparvovirus is a novel type of feline parvovirus. The present study aims to establish a...
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Simultaneous detection of feline parvovirus and feline bocavirus using SYBR Green I-based duplex real-time polymerase chain reaction
Since both feline parvovirus (FPV) and feline bocavirus (FBoV) can cause diarrhea in cats, it is difficult to distinguish them clinically. This study...
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Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
AbstractThe pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For develo** countries where vaccines are still in...
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One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India
Rapid, sensitive, and reliable laboratory detection of foot-and-mouth disease virus (FMDV) infection is essential for containing and controlling...
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qPCR Assay for the Detection of Hepatotoxigenic Cyanobacteria
Harmful algal blooms are contaminating fresh and marine waterbodies. The frequency and toxicity of these algal blooms are predicted to rise at an...
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Comprehensive and species-specific detection of mackerels of the genus Scomber in processed foods using SYBR Green-based real-time PCR
The genus Scomber , commonly known as mackerel, is recognized as a potential allergenic fish species, necessitating the assessment of Scomber species...
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Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants
The emergence of circulating DNA analysis in blood during the past decade has responded to the need for noninvasive alternatives to classical tissue...
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qPCR Assay in sxtA Gene in Saxitoxin-Producing Cyanobacteria
Saxitoxin, as one type of paralytic shellfish toxin, can bind to sodium channels and modulate the flux of sodium in nerve and muscle cells....
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Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus
The Indian citrus ringspot virus (ICRSV) that causes ringspot disease, especially to ‘Kinnow mandarin’ hampers the sustainability of crop production....
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Real-Time or Quantitative PCR
Real-time PCR is employed for the estimation of differential mRNA expression profile of genes. It is the quantitative expression of a series of genes...
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A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus
Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal...
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Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection
Porcine deltacoronavirus (PDCoV) is a newly emerged swine enteric coronavirus that poses a major threat to the pig industry. Currently, there is no...
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Magnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus
African swine fever (ASF) is a deadly disease in swine caused by African swine fever virus (ASFV). The global spread of ASFV has resulted in...
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Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis
TaqMan probe-based commercial real-time (RT) PCR kits are expensive but most frequently used in COVID-19 diagnosis. The unprecedented scale of...
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Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction...
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Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions
Emerging viruses pose significant threats to human health and the global economy. In the past two decades, three different coronaviruses have emerged...
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Establishment of a real-time fluorescent quantitative PCR detection method and phylogenetic analysis of BoAHV-1
BackgroundInfectious bovine rhinotracheitis (IBR), caused by Bovine alphaherpesvirus-1 (BoAHV-1), is an acute, highly contagious disease primarily...
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A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae
Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds...
