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Map** periplasmic binding protein oligosaccharide recognition with neutron crystallography
Numerous studies have shown how periplasmic binding proteins (PBPs) bind substrates with exquisite specificity, even distinguishing between sugar...
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Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation
Periplasmic solute-binding proteins (SBPs) specific for chitooligosaccharides, (GlcNAc) n (n = 2, 3, 4, 5 and 6), are involved in the uptake of...
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Protein-Protein Binding Kinetics by Biolayer Interferometry
The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the...
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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria
The bacterial flagellum, which facilitates motility, is composed of ~20 structural proteins organized into a long extracellular filament connected to...
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Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
Extracellular electron transfer (EET) via microbial nanowires drives globally-important environmental processes and biotechnological applications for...
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Conformational Investigation of the Asymmetric Periplasmic Domains of E. coli LptB2FGC Using SDSL CW EPR Spectroscopy
The majority of pathogenic Gram-negative bacteria benefit from intrinsic antibiotic resistance, attributed primarily to the lipopolysaccharide (LPS)...
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Structure and mechanism of a tripartite ATP-independent periplasmic TRAP transporter
In bacteria and archaea, tripartite ATP-independent periplasmic (TRAP) transporters uptake essential nutrients. TRAP transporters receive their...
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Conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP
The tripartite ATP-independent periplasmic (TRAP) transporters use an extra cytoplasmic substrate binding protein (SBP) to transport a wide variety...
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Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system
EfeUOB/M has been characterised in Pseudomonas syringae pathovar. syringae as a novel type of ferrous-iron transporter, consisting of an...
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Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter
Tripartite ATP-independent periplasmic (TRAP) transporters are found widely in bacteria and archaea and consist of three structural domains, a...
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The critical role of a conserved lysine residue in periplasmic nitrate reductase catalyzed reactions
Periplasmic nitrate reductase NapA from Campylobacter jejuni ( C. jejuni ) contains a molybdenum cofactor (Moco) and a 4Fe–4S cluster and catalyzes the...
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Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria depends on delivery of unfolded OMPs to the...
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A role for the periplasmic adaptor protein AcrA in vetting substrate access to the RND efflux transporter AcrB
Tripartite resistance-nodulation-division (RND) efflux pumps, such as AcrAB-TolC of Salmonella Typhimurium, contribute to antibiotic resistance and...
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Disulfide-compatible phage-assisted continuous evolution in the periplasmic space
The directed evolution of antibodies has yielded important research tools and human therapeutics. The dependence of many antibodies on disulfide...
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S288T mutation altering MmpL3 periplasmic domain channel and H-bond network: a novel dual drug resistance mechanism
ContextMycobacterial membrane proteins Large 3 (MmpL3) is responsible for the transport of mycobacterial acids out of cell membrane to form cell...
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Protein–Protein Interactions: Oxidative Bacterial Two Hybrid
Protein–protein interaction studies are essential to understand how proteins organize themselves into interaction networks and thus influence...
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Directed evolution of tripartite ATP-independent periplasmic transporter for 3-Hydroxypropionate biosynthesis
AbstractOur previous study’s introduction of the malonic acid assimilation pathway into Escherichia coli enabled biosynthesis of 3-Hydroxypropionate...
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A periplasmic cinched protein is required for siderophore secretion and virulence of Mycobacterium tuberculosis
Iron is essential for growth of Mycobacterium tuberculosis , the causative agent of tuberculosis. To acquire iron from the host, M. tuberculosis uses...
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Engineering of Sensory Proteins with New Ligand-Binding Capacities
Biosensors based on whole cell living bacteria or their isolated components mostly use the capacity of natural protein classes to “sense,” in other...
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Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant buforin I expressed in periplasmic space of Escherichia coli
The lack of cost-effective methods for producing antimicrobial peptides has made it impossible to use their high potential as a new and powerful...
