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Calcium signaling positively regulates cellulase translation and secretion in a Clr-2-overexpressing, catabolically derepressed strain of Penicillium funiculosum
BackgroundLow-cost cellulase production is vital to sustainable second-generation biorefineries. The catabolically derepressed strain of Penicillium...
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Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30
BackgroundThe fungus Trichoderma reesei is one of the most used industrial cellulase producers due to its high capacity of protein secretion....
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Cellular ATP redistribution achieved by deleting Tgparp improves lignocellulose utilization of Trichoderma under heat stress
BackgroundThermotolerance is widely acknowledged as a pivotal factor for fungal survival across diverse habitats. Heat stress induces a cascade of...
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An insight into transcriptome of Cyathus bulleri for lignocellulase expression on wheat bran
To identify enzymes that can be effectively used for hydrolysis of lignocellulosic biomass, an attractive carbon source in biorefineries,...
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Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants
BackgroundGlucoamylase is an important enzyme for starch saccharification in the food and biofuel industries and mainly produced from mesophilic...
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Comparative genomic and transcriptome analysis of Bacillus velezensis CL-4 fermented corn germ meal
Bacillus, an excellent organic-degrading agent, can degrade lignocellulose. Notably, some B. velezensis strains encode lignocellulases. However,...
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Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366
Cellulase production by fungi is tightly regulated in response to environmental cues, and understanding this mechanism is a key pre-requisite in the...
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An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production
BackgroundThe CRISPR/Cas9 technology is being employed as a convenient tool for genetic engineering of the industrially important filamentous fungus T...
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The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei
Protein methylation is a commonly posttranslational modification of transcriptional regulators to fine-tune protein function, however, whether this...
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Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide
The mixture of glucose and β-disaccharide (MGD) synthesized by transglycosylation of glucose as a low-cost soluble carbon source can efficiently...
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Exploring the multi-level regulation of lignocellulases in the filamentous fungus Trichoderma guizhouense NJAU4742 from an omics perspective
BackgroundFilamentous fungi are highly efficient at deconstructing plant biomass by secreting a variety of enzymes, but the complex enzymatic...
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Cultivation methods, characterization, and biocatalytic potential of organic solid waste degrading bacteria isolated from sugarcane rhizospheric soil and compost
The study aimed to investigate organic waste-degrading microbial communities in compost and sugarcane rhizospheric soil from Kodinar, Gujarat, India...
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Rapid Deconstruction of Cotton, Coir, Areca, and Banana Fibers Recalcitrant Structure Using a Bacterial Consortium with Enhanced Saccharification
This study aims to establish a bacterial consortium to deconstruct the coir, areca, banana, and cotton fibers recalcitrant structure. Hence,...
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Expression of microbial lipase in filamentous fungus Aspergillus niger: a review
Lipase has high economic importance and is widely used in biodiesel, food, detergents, cosmetics, and pharmaceutical industries. The rapid...
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The cell functions of phospholipase C-1, Ca2+/H+ exchanger-1, and secretory phospholipase A2 in tolerance to stress conditions and cellulose degradation in Neurospora crassa
We investigated the cell functions of the Ca 2+ signaling genes phospholipase C-1 ( plc-1 ), Ca 2+ / H + exchanger ( cpe-1 ), and secretory phospholipase A 2 ( splA...
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A review of additives use in straw composting
Straw composting is not only a process of decomposition and re-synthesis of organic matter, but also a process of harmless treatment, avoiding air...
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Cellulose-degrading enzymes: key players in biorefinery development
Saccharification recalcitrance significantly limits the conversion of lignocellulosic biomass to valuable end products. The intricate arrangement...
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Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiber
Penicillium o xa licum engineered strain DB2 and its mutant strains with multiple regulatory modules were constructed. Mutant strain RE-4-2 with two...
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Nitrogen sources alter ligninase and cellulase activities of thermophilic fungi isolated from compost and vermicompost
Fungi harboring lignocellulolytic activity accelerate the composting process of agricultural wastes; however, using thermophilic fungal isolates for...
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The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei
BackgroundLignocellulose is the most abundant renewable resource in the world and has attracted widespread attention. It can be hydrolyzed into...
