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Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A
KIF1A is a neuron-specific member of the kinesin-3 family of microtubule (MT) plus-end-directed motor proteins. It powers the migration of nuclei in...
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Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips
Optical traps have enabled foundational studies of how mechanoenzymes such as kinesins and dynein motors walk along microtubules, how myosins move...
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Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies
Cytoskeletal motor proteins are essential molecular machines that hydrolyze ATP to generate force and motion along cytoskeletal filaments. Members of...
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Extraction, Purification, and Small-Angle X-ray Scattering Analysis of the YsxC GTPase of Staphylococcus aureus
AbstractStudy of the structures of various biological macromolecules is one of the main directions that are intensively developed using physical...
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Solid-state NMR backbone chemical shift assignments of α-synuclein amyloid fibrils at fast MAS regime
The α-synuclein (α-syn) amyloid fibrils are involved in various neurogenerative diseases. Solid-state NMR (ssNMR) has been showed as a powerful tool...
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Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors
The cytoskeletal motors myosin, kinesin, and dynein and their corresponding tracks, actin and microtubules, are force generating ATPases responsible...
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Development of visible light-driven nanorod-like MoO3@ZnO nanocomposite: an affordable catalyst for the degradation of organic dye moiety
An effective visible light catalyst was successfully fabricated from a nanorod-like MoO 3 /ZnO (MZ) nanocomposite using a simple hydrothermal method...
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Dreams of Declassification: The Early Cold War Quest for Nuclear Knowledge in The Netherlands and Norway
This article seeks to explore the relation between nuclear physics and secrecy in early Cold War Europe. After World War II, nuclear physics...
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Backbone and side chain resonance assignment of the intrinsically disordered human DBNDD1 protein
The dysbindin domain-containing protein 1 (DBNDD1) is a conserved protein among higher eukaryotes whose structure and function are poorly...
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Resonance assignment and secondary structure of DbpA protein from the European species, Borrelia afzelii
Decorin binding proteins (Dbps) mediate attachment of spirochetes in host organisms during the early stages of Lyme disease infection. Previously,...
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The Soret coefficient of human low-density lipoprotein in solution: a thermophilic behavior
Thermodiffusion, or Soret effect, is the physical phenomenon of matter gradients originated by the migration of chemical species induced by thermal...
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Introduction
Since the beginning of the 21st century, nanoscience and nanotechnology have advanced significantly. A variety of nanostructures and devices based on...
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Backbone assignment of crystalline E. coli maltose binding protein
The E.coli maltose binding protein (MBP) is a 42.5 kDa molecule widely employed in many biotechnology applications. Because of its molecular size, it...
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Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa whereas magic-angle-spinning (MAS) solid-state NMR...
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Preparation and Characterization of Influenza Virosomes Using Nonionic, Dialyazble Phospholipid for Efficient Membrane Solubilization and Reconstitution
The present study aimed to formulate a novel strategy to prepare influenza A/H1N1 virosomes using 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)...
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Activity and Catalytic Characteristics of Rat Liver Mitochondrial Succinate Dehydrogenase under Moderate Hypothermia
AbstractWe have previously shown that short-term moderate (30°C) hypothermia contributed to a significant increase in the intensity of free radical...
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Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy
Amyloid proteins are associated with a broad spectrum of neurodegenerative diseases. However, it remains a grand challenge to extract molecular...
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Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril
The aggregation of antibody light chains is linked to systemic light chain (AL) amyloidosis, a disease where amyloid deposits frequently affect the...
