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Label-Free Quantitation of Endogenous Peptides
Liquid chromatography–mass spectrometry (LC-MS)-based peptidomics methods allow for the detection and identification of many peptides in a complex...
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Absolute quantitation of human wild-type DNAI1 protein in lung tissue using a nanoLC-PRM-MS-based targeted proteomics approach coupled with immunoprecipitation
BackgroundDynein axonemal intermediate chain 1 protein (DNAI1) plays an essential role in cilia structure and function, while its mutations lead to...
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Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues
Mouse is the mammalian model of choice to study human health and disease due to its size, ease of breeding and the natural occurrence of conditions...
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An Updated Guide to the Identification, Quantitation, and Imaging of the Crustacean Neuropeptidome
Crustaceans serve as a useful, simplified model for studying peptides and neuromodulation, as they contain numerous neuropeptide homologs to mammals...
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Quantitation of DNA Using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit
Following the isolation of deoxyribonucleic acid (DNA) from biological samples, the quantitation of amplifiable human DNA is a critical next step in...
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Ultrasensitive quantitation of FLT3-ITD mutation in patients with acute myeloid leukemia using ddPCR
BackgroundFLT3 -ITD mutations occur in 45–50% of cytogenetically normal AML patients. Conventional fragment analysis using capillary electrophoresis...
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Quantitation of ER Morphology and Dynamics
The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is...
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Albumen and Yolk Plasma Peptidomics for the Identification and Quantitation of Bioactive Molecules and the Quality Control of Hen Egg Products
Hen eggs and the corresponding food products are essential components of human diet. In addition to supplying basic nutrients, they contain...
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Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane
It is a challenging task to quantify mucin using conventional protein quantification methods due to the large number of glycans attached to the...
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Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry
Mucins MUC5AC and MUC5B are large glycoproteins that play an essential role in the innate defense of epithelial surfaces and their quantitation in...
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Mitochondrial interactome quantitation reveals structural changes in metabolic machinery in the failing murine heart
Advancements in cross-linking mass spectrometry bridge the gap between purified systems and native tissue environments, allowing the detection of...
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Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research
Current research is focusing on ribosome heterogeneityRibosome heterogeneity as a response to changing environmental conditions and stressesStresses....
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Quantitation of Glutamine Synthetase 1 Activity in Drosophila melanogaster
Protocols to assay the activity of glutamine synthetase (GS) are presented as they have been used in our laboratory to correlate the expression...
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A Modified Differential Centrifugation Protocol for Isolation and Quantitation of Extracellular Heat Shock Protein 90 (eHsp90)
Studies of the past 15 years have revealed a critical role for extracellular heat shock protein 90alpha (eHsp90α) in the development of several human...
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Quantitation of DNA-Encoded Libraries by qPCR
DNA-encoded library (DEL) yields can be easily measured throughout the selection process using the quantitative polymerase chain reaction (qPCR)...
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Absolute Quantitation of GTPase Protein Abundance
Ras proteins and other small molecular weight GTPases are molecular switches controlling a wide range of cellular functions. High homology and...
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Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MS
Oxidation of glutathione (GSH) to its disulfide dimer (GSSG) is the major mechanism by which cells balance reactive oxygen species (ROS) and mitigate...
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Identification, map** and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
We describe an analytical method for the identification, map** and relative quantitation of glycopeptides from SARS-CoV-2 Spike protein. The method...
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Discovering Protein–Protein Interactions using Co-Fractionation-Mass Spectrometry with Label-Free Quantitation
Proteins generally achieve their functions through interactions with other proteins, so being able to determine which proteins interact with which...
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Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter
The transforming growth factor-β (TGF-β) is a multifunctional cytokine critical for embryogenesis and tissue homeostasis. Alterations in TGF-β...