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Ras Activity and Signaling

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ignacio Rubio

    1. Department Anesthesiology and Intensive Care, Jena University Hospital, Jena, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Ian Prior

    1. Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2262)

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About this book

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Generic Ras Biology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Ras Variant Biology and Contributions to Human Disease

      • Ian Prior
      Pages 3-18

    3. Regulation of the Small GTPase Ras and Its Relevance to Human Disease

      • Kayla R. Kulhanek, Jeroen P. Roose, Ignacio Rubio
      Pages 19-43

  3. Methods in Ras Biochemistry

    1. Front Matter

      Pages 45-45
      Download chapter PDF

    2. Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry

      • Lauren M. Adams, Caroline J. DeHart, Neil L. Kelleher
      Pages 47-64

    3. Absolute Quantitation of GTPase Protein Abundance

      • Fiona E. Hood, Yasmina M. Sahraoui, Rosalind E. Jenkins, Ian Prior
      Pages 65-90

    4. Validation of Isoform- and Mutation-Specific RAS Antibodies

      • Andrew M. Waters, Channing J. Der
      Pages 91-103

    5. Production and Membrane Binding of N-Terminally Acetylated, C-Terminally Farnesylated and Carboxymethylated KRAS4b

      • Simon Messing, Constance Agamasu, Matt Drew, Caroline J. DeHart, Andrew G. Stephen, William K. Gillette
      Pages 105-116

    6. Active GTPase Pulldown Protocol

      • Martin J. Baker, Ignacio Rubio
      Pages 117-135

    7. Methods to Monitor Ras Activation State

      • Kari Kopra, Harri Härmä
      Pages 137-167

    8. NMR Detection Methods for Profiling RAS Nucleotide Cycling

      • Ryan C. Killoran, Matthew J. Smith
      Pages 169-182

  4. Methods in Ras Processing, Trafficking, and Localization

    1. Front Matter

      Pages 183-183
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    2. Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations

      • Orit Gutman, Marcelo Ehrlich, Yoav I. Henis
      Pages 185-197

    3. Understanding Ras Spatial Cycles Through Reaction-Diffusion Simulations

      • Malte Schmick, Philippe I. H. Bastiaens
      Pages 199-215

    4. Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets

      • Yong Zhou, John F. Hancock
      Pages 217-232

    5. FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage

      • Hanna Parkkola, Farid Ahmad Siddiqui, Christina Oetken-Lindholm, Daniel Abankwa
      Pages 233-250

    6. Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry

      • Natividad R. Fuentes, Michael L. Salinas, **aoli Wang, Yang-Yi Fan, Robert S. Chapkin
      Pages 251-258

    7. Spatiotemporal Imaging of Small GTPase Activity Using Conformational Sensors for GTPase Activity (COSGA)

      • Yao-Wen Wu
      Pages 259-267

  5. Methods in Ras Signaling and Inhibition

    1. Front Matter

      Pages 269-269
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Editors and Affiliations

  • Department Anesthesiology and Intensive Care, Jena University Hospital, Jena, Germany

    Ignacio Rubio

  • Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK

    Ian Prior

Bibliographic Information

  • Book Title: Ras Activity and Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Ignacio Rubio, Ian Prior

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1190-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1189-0Published: 12 May 2021

  • Softcover ISBN: 978-1-0716-1192-0Published: 12 May 2022

  • eBook ISBN: 978-1-0716-1190-6Published: 11 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 439

  • Number of Illustrations: 29 b/w illustrations, 57 illustrations in colour

  • Topics: Protein Science

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