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Hsp104p: a protein disaggregase
All newly synthesized proteins must fold to their correct native conformation in order to function. That protein folding in the crowded...
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PA28γ–20S proteasome is a proteolytic complex committed to degrade unfolded proteins
PA28γ is a nuclear activator of the 20S proteasome that, unlike the 19S regulatory particle, stimulates hydrolysis of several substrates in an ATP-...
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Resolving chaperone-assisted protein folding on the ribosome at the peptide level
Protein folding in vivo begins during synthesis on the ribosome and is modulated by molecular chaperones that engage the nascent polypeptide. How...
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Mutational Analysis of Protein Folding Transition States: Phi Values
The analysis of protein folding reactions by monitoring the kinetic effects of specifically designed single-point mutations, the so-termed phi-value...
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Biophysical studies of amorphous protein aggregation and in vivo immunogenicity
Amorphous protein aggregates are oligomers that lack specific, high-order structures. Soluble amorphous aggregates are smaller than ~1 µm. Despite...
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Protein nanocondensates: the next frontier
Over the past decade, myriads of studies have highlighted the central role of protein condensation in subcellular compartmentalization and...
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Assessment of Heat-Shock Protein Hsp70 Colocalization with Markers of Tumor Stem-Like Cells
AbstractThe search of biomarkers for early diagnosis and theranostics of glioblastoma multiforme (GBM) due to the aggressiveness of the disease is an...
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Reflecting on mutational and biophysical analysis of Gaussia princeps Luciferase from a structural perspective: a unique bioluminescent enzyme
Gaussia princeps luciferase (GLuc 18.2 kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizing coelenterazine...
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Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Yeast surface display has proven to be a powerful tool for the discovery of antibodies and other novel binding proteins and for engineering the...
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Energetic Aspects of Protein–Protein Interactions (PPIs)
The increasing demand to build holistic models of protein–protein interactions in the cell requires understanding them at the structural level....
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Predicting Protein Conformational Disorder and Disordered Binding Sites
In the last two decades it has become increasingly evident that a large number of proteins adopt either a fully or a partially disordered...
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Recombinant Expression and Overproduction of Transmembrane β-Barrel Proteins
Transmembrane β-barrel proteins reside in the outer membrane of Gram-negative bacteria and are thus in direct contact with the environment. Because...
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Assessing and Improving Protein Sample Quality
One essential prerequisite of any experiment involving a purified protein, such as interaction studies or structural and biophysical...
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Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12
The synaptonemal complex (SC) is a supramolecular protein scaffold that mediates chromosome synapsis and facilitates crossing over during meiosis. In...
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HSF1, Aging, and Neurodegeneration
Heat shock factor 1 (HSF1) is a master transcription regulator that mediates the induction of heat shock protein chaperones for quality control (QC)...
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Free-energy landscapes of membrane co-translocational protein unfolding
Protein post-translational translocation is found at the plasma membrane of prokaryotes and protein import into organellae. Translocon structures are...
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Evaluation of a bacterial group 1 LEA protein as an enzyme protectant from stress-induced inactivation
AbstractLate embryogenesis abundant (LEA) proteins are hydrophilic proteins that lack a well-ordered tertiary structure and accumulate to high levels...
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De novo design of tyrosine and serine kinase-driven protein switches
Kinases play central roles in signaling cascades, relaying information from the outside to the inside of mammalian cells. De novo designed protein...
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α-Linolenic Acid Vesicles-Mediated Tau Internalization in Microglia
In Alzheimer’s disease, the synaptic loss is prominent due to the accumulation of Amyloid βeta (Aβ) protein in synapses, which affect...