Abstract
The search of biomarkers for early diagnosis and theranostics of glioblastoma multiforme (GBM) due to the aggressiveness of the disease is an urgent needed. In the present work, the expression of the heat-shock protein Hsp70 was detected and a quantitative assessment of its colocalization with markers of tumor stem-like cells (TSLCs) was made using the multiplex method of analysis of histological preparations of GBM. Cells expressing Hsp70 are uniformly distribution in the tumor and have colocalization with Nestin and Sox2, indicate the promise of using Hsp70 as a target for targeted therapy of malignant brain tumors.
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ACKNOWLEDGMENTS
The authors express their deep gratitude to the BioLine LLC Group for the reagents and equipment provided for the work, as well as to Darya Dyakova, an employee of this company, for her help in preparing and analyzing preparations.
Funding
This work was supported by the Russian Foundation for Basic Research, project no. 19-58-55001.
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Statement of compliance with standards of research involving humans as subjects. The protocol of the clinical trial was approved by the Ethics Committee of the Almazov Federal Medical Research Center. All patients signed an informed consent to provide material for research.
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Abbreviations: IHC, immunohistochemistry; IF, immunofluorescence; GBM, glioblastoma multiforme; TSLCs, tumor stem-like cells; DAB, 3,3'-diaminobenzidine; HSP, heat-shock protein; PBS, phosphate-buffered saline solution.
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Yudintceva, N.M., Mikhrina, A.L., Nechaeva, A.S. et al. Assessment of Heat-Shock Protein Hsp70 Colocalization with Markers of Tumor Stem-Like Cells. Cell Tiss. Biol. 16, 459–464 (2022). https://doi.org/10.1134/S1990519X22050108
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DOI: https://doi.org/10.1134/S1990519X22050108